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Literature summary for 7.4.2.8 extracted from
- Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids (2007), EMBO J., 26, 19-27.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cardiolipin | via the EpsE/cyto-EpsL complex | Vibrio cholerae serotype O1 |  |
| phosphatidylglycerol | via the EpsE/cyto-EpsL complex | Vibrio cholerae serotype O1 | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmids pEpsE/EpsL(1-253)His6 and pEpsE/EpsL(1-242)His6 using pET21d+ are constructed, for expression of monomeric EpsE the vector GST-EpsE is constructed | Vibrio cholerae serotype O1 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K270A | EpsE mutant | Vibrio cholerae serotype O1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Vibrio cholerae serotype O1 | - |
ADP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae serotype O1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| EpsE is copurified with the cytoplasmic domain of EpsL to test whether the ATPase activity of EpsE can be modulated by EpsL, complexes are purified using metal affinity chromatography and gel filtration, monomeric EpsE is obtained following thrombin cleavage of a GST-EpsE fusion protein | Vibrio cholerae serotype O1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Vibrio cholerae serotype O1 | ADP + phosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EpsE | cytoplasmic component of the type II secretion system | Vibrio cholerae serotype O1 |
| protein-secreting ATPase | - |
Vibrio cholerae serotype O1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
ATPase activity assay | Vibrio cholerae serotype O1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
ATPase activity assay | Vibrio cholerae serotype O1 |
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Release 2023.1 (January 2023)
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