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Literature summary for 7.4.2.8 extracted from
- Type III protein translocase: HrcN is a peripheral ATPase that is activated by oligomerization (2003), J. Biol. Chem., 278, 25816-25824.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant decahistidinyl derivative | Pseudomonas syringae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.114 | - |
ATP | pH 8.0, 30°C, monomeric form | Pseudomonas syringae |  |
| 1.3 | - |
ATP | pH 8.0, 30°C, dodecameric form | Pseudomonas syringae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | peripheral membrane protein, associates with inner membrane | Pseudomonas syringae | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM, required, best activating kation | Pseudomonas syringae | |
| Mn2+ | 5 mM, can partly replace Mg2+ | Pseudomonas syringae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
sedimentation analysis | Pseudomonas syringae |
| 300000 | - |
sedimentation analysis | Pseudomonas syringae |
| 575000 | - |
sedimentation analysis, predominant form | Pseudomonas syringae |
| 3500000 | - |
sedimentation analysis | Pseudomonas syringae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas syringae | Q8RK01 | pathovar phaseolica | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant decahistidinyl derivative | Pseudomonas syringae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Pseudomonas syringae | ADP + phosphate | - |
? | |
| ATP + H2O + cellular protein[side 1] | - |
Pseudomonas syringae | ADP + phosphate + cellular protein[side 2] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | 12 * 48000, SDS-PAGE, predominant form at membrane, activity increases 700fold over monomeric form | Pseudomonas syringae |
| hexamer | 6 * 48000, SDS-PAGE | Pseudomonas syringae |
| monomer | 1 * 48000, SDS-PAGE | Pseudomonas syringae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HrcN | - |
Pseudomonas syringae |
| TTS ATPase | - |
Pseudomonas syringae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
- |
Pseudomonas syringae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.052 | - |
ATP | pH 8.0, 30°C, monomeric form | Pseudomonas syringae | |
| 447 | - |
ATP | pH 8.0, 30°C, dodecameric form | Pseudomonas syringae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas syringae |
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Release 2023.1 (January 2023)
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