Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.4.2.1 extracted from

  • Van Zeebroeck, G.; Bonini, B.M.; Versele, M.; Thevelein, J.M.
    Transport and signaling via the amino acid binding site of the yeast Gap1 amino acid transceptor (2009), Nat. Chem. Biol., 5, 45-52.
    View publication on PubMed
Search for more literature for 7.4.2.1

Protein Variants

Protein Variants Comment Organism
I394 site-directed mutagenesis, the mutation strongly affects both transport and signaling with citrulline and other amino acids Saccharomyces cerevisiae
additional information mutation of each of the amino acid residues V381, I382, L383, I384, A385, L387, S388, V389, G390, N391, A393, A396, S398, R399, T400, M401, A403, l404, and A405 to cysteine does not affect transport or signaling by the enzyme Saccharomyces cerevisiae
S392C site-directed mutagenesis, the mutation strongly affects both transport and signaling with citrulline and other amino acids Saccharomyces cerevisiae
V386C site-directed mutagenesis, the mutation strongly affects both transport and signaling with citrulline and other amino acids Saccharomyces cerevisiae
V402C site-directed mutagenesis, the mutation strongly affects both transport and signaling with citrulline and other amino acids Saccharomyces cerevisiae
Y395C site-directed mutagenesis, the mutation strongly affects both transport and signaling with citrulline and other amino acids Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Arg-Phe-Phe noncompetitive Saccharomyces cerevisiae
L-Asp-gamma-L-Phe competitive Saccharomyces cerevisiae
L-Leu-Gly competitive Saccharomyces cerevisiae
Leu-Met-NH2
-
 Saccharomyces cerevisiae
additional information inhibition of agonist activity by the inhibitor molecules, overveiw Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Saccharomyces cerevisiae 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + polar amino acid/out Saccharomyces cerevisiae
-
 ADP + phosphate + polar amino acid/in
-
 ?
additional information Saccharomyces cerevisiae Gap1 is a transporting amino acid transceptor that triggers the activation of the protein kinase A pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + citrulline/out
-
 Saccharomyces cerevisiae ADP + phosphate + citrulline/in
-
 ?
ATP + H2O + polar amino acid/out
-
 Saccharomyces cerevisiae ADP + phosphate + polar amino acid/in
-
 ?
ATP + H2O + polar amino acid/out Ser388 and Val389 are exposed into the amino acid binding site for transport, also used by agonist action for signaling Saccharomyces cerevisiae ADP + phosphate + polar amino acid/in
-
 ?
additional information Gap1 is a transporting amino acid transceptor that triggers the activation of the protein kinase A pathway Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
amino acid transceptor
-
 Saccharomyces cerevisiae
GAP1
-
 Saccharomyces cerevisiae
transporting amino acid transceptor
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.105
-
 L-Asp-gamma-L-Phe
-
 Saccharomyces cerevisiae
0.19
-
 L-Leu-Gly
-
 Saccharomyces cerevisiae