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Literature summary for 7.3.2.1 extracted from

  • Lazard, M.; Blanquet, S.; Fisicaro, P.; Labarraque, G.; Plateau, P.
    Uptake of selenite by Saccharomyces cerevisiae involves the high- and low-affinity orthophosphate transporters (2011), J. Biol. Chem., 285, 32029-32037.
    View publication on PubMed
Search for more literature for 7.3.2.1

Inhibitors

Inhibitors Comment Organism Structure
Selenite competitively inhibits phosphate uptake; competitively inhibits phosphate uptake; competitively inhibits phosphate uptake; competitively inhibits phosphate uptake Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 phosphate pH 6.0, 30°C, Vmax: 860 pmol of phosphate incorporated per min/OD650 Saccharomyces cerevisiae
0.025
-
 phosphate wild-type, pH 6.0, 30°C, Vmax: 440 pmol of phosphate incorporated per min/OD650 Saccharomyces cerevisiae
0.025
-
 phosphate using triple mutant DELTApho87-DELTApho90-DELTApho91, pH 6.0, 30°C, Vmax: 2000 pmol of phosphate incorporated per min/OD650 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P25297
-
-
Saccharomyces cerevisiae P25360
-
-
Saccharomyces cerevisiae P39535
-
-
Saccharomyces cerevisiae P27514
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphate/out
-
 Saccharomyces cerevisiae ADP + phosphate + phosphate/in
-
 ?

Synonyms

Synonyms Comment Organism
high-affinity phosphate transporter Pho84p
-
 Saccharomyces cerevisiae
low-affinity phosphate transporter Pho87p
-
 Saccharomyces cerevisiae
low-affinity phosphate transporter Pho90p
-
 Saccharomyces cerevisiae
low-affinity phosphate transporter Pho91p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.6
-
 Selenite
-
 Saccharomyces cerevisiae
5.5
-
 Selenite using triple mutant DELTApho87-DELTApho90-DELTApho91 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction selenite uptake is reduced in a DELTA pho84 mutant Saccharomyces cerevisiae
malfunction a triple mutant consisting of deleted low-affinity transporters Pho87, Pho90 and Pho91 is slightly more sensitive to selenite than the wild-type strain. Single mutants do not show any difference to the wild-type stain Saccharomyces cerevisiae
malfunction a triple mutant consisting of deleted low-affinity transporters Pho87, Pho90 and Pho91 is slightly more sensitive to selenite than the wild-type strain. Single mutants do not show any difference to the wild-type strain Saccharomyces cerevisiae
physiological function when cells are grown at low phosphate concentrations, the high-affinity phosphate transporter Pho84p is the major contributor to selenite uptake. Pho84p is very selective for phosphate as compared to selenite Saccharomyces cerevisiae
physiological function when phosphate is abundant, selenite is internalized through the low-affinity phosphate transporters (Pho87p, Pho90p, and Pho91p). Low-affinity transporters discriminate less efficiently between selenite and phosphate Saccharomyces cerevisiae