Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression analysis, functional overexpression of the enzyme as His6-Xpress-ANTR1-FLAG fusion protein in Escherichia coli, the recombinantly expressed Arabidopsis ANTR1 facilitates Na+-dependent phosphate transport into Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose 6-phosphate | 37.5% inhibition at 2.5 mM | Arabidopsis thaliana | |
diphosphate | 26% inhibition at 2.5 mM | Arabidopsis thaliana | |
glutamate | 56% inhibition at 2.5 mM | Arabidopsis thaliana | |
Li+ | indirect inhibition | Arabidopsis thaliana | |
methylphosphonate | 79% inhibition at 2.5 mM | Arabidopsis thaliana | |
phosphate | substrate inhibition, 85.5% inhibition at 2.5 mM | Arabidopsis thaliana | |
sulfate | 21% inhibition at 2.5 mM | Arabidopsis thaliana | |
vanadate | 26% inhibition at 2.5 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0787 | - |
phosphate | pH 6.5, 25°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the enzyme contains a putative chloroplast transit peptide with a cleavage site between amino acids 59-60 | Arabidopsis thaliana | 9507 | - |
thylakoid membrane | - |
Arabidopsis thaliana | 42651 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates 5fold | Arabidopsis thaliana | |
Na+ | required only at pH higher than 5.5, stimulates 15fold | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE | Arabidopsis thaliana |
50700 | - |
x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE | Arabidopsis thaliana |
56500 | - |
x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | Arabidopsis thaliana | ANTR1 functions as a Na+-dependent phosphate transporter | ADP + phosphate + phosphate/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O82390 | full-length enzyme; cv. Columbia | - |
Purification (Comment) | Organism |
---|---|
native enzyme partially by preparation of chloroplasts and thylakoid membranes | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower bud | high expression level | Arabidopsis thaliana | - |
leaf | high expression in mature leaves, lower expression in senescent leaves | Arabidopsis thaliana | - |
additional information | no expression of ANTR1 in roots | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | ANTR1 functions as a Na+-dependent phosphate transporter | Arabidopsis thaliana | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | phosphate is the preferred substrate | Arabidopsis thaliana | ADP + phosphate + phosphate/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
anion transporter 1 | - |
Arabidopsis thaliana |
ANTR1 | - |
Arabidopsis thaliana |
Na+-dependent inorganic phosphate transporter | - |
Arabidopsis thaliana |
Na+-dependent Pi transporter | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |