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Literature summary for 7.3.2.1 extracted from

  • Pavon, L.R.; Lundh, F.; Lundin, B.; Mishra, A.; Persson, B.L.; Spetea, C.
    Arabidopsis ANTR1 is a thylakoid Na+-dependent phosphate transporter: functional characterization in Escherichia coli (2008), J. Biol. Chem., 283, 13520-13527.
    View publication on PubMed
Search for more literature for 7.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression analysis, functional overexpression of the enzyme as His6-Xpress-ANTR1-FLAG fusion protein in Escherichia coli, the recombinantly expressed Arabidopsis ANTR1 facilitates Na+-dependent phosphate transport into Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 6-phosphate 37.5% inhibition at 2.5 mM Arabidopsis thaliana
diphosphate 26% inhibition at 2.5 mM Arabidopsis thaliana
glutamate 56% inhibition at 2.5 mM Arabidopsis thaliana
Li+ indirect inhibition Arabidopsis thaliana
methylphosphonate 79% inhibition at 2.5 mM Arabidopsis thaliana
phosphate substrate inhibition, 85.5% inhibition at 2.5 mM Arabidopsis thaliana
sulfate 21% inhibition at 2.5 mM Arabidopsis thaliana
vanadate 26% inhibition at 2.5 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0787
-
 phosphate pH 6.5, 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the enzyme contains a putative chloroplast transit peptide with a cleavage site between amino acids 59-60 Arabidopsis thaliana 9507
-
thylakoid membrane
-
 Arabidopsis thaliana 42651
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulates 5fold Arabidopsis thaliana
Na+ required only at pH higher than 5.5, stimulates 15fold Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE Arabidopsis thaliana
50700
-
 x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE Arabidopsis thaliana
56500
-
 x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + phosphate/out Arabidopsis thaliana ANTR1 functions as a Na+-dependent phosphate transporter ADP + phosphate + phosphate/in
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O82390 full-length enzyme; cv. Columbia
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by preparation of chloroplasts and thylakoid membranes Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower bud high expression level Arabidopsis thaliana
-
leaf high expression in mature leaves, lower expression in senescent leaves Arabidopsis thaliana
-
additional information no expression of ANTR1 in roots Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphate/out ANTR1 functions as a Na+-dependent phosphate transporter Arabidopsis thaliana ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out phosphate is the preferred substrate Arabidopsis thaliana ADP + phosphate + phosphate/in
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, about, thylakoid membrane enzyme, SDS-PAGE, x * 56500, unprocessed enzyme containing the chloroplast transit peptide, SDS-PAGE, x * 50700, processed enzyme without transit peptide, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
anion transporter 1
-
 Arabidopsis thaliana
ANTR1
-
 Arabidopsis thaliana
Na+-dependent inorganic phosphate transporter
-
 Arabidopsis thaliana
Na+-dependent Pi transporter
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana