Cloned (Comment) | Organism |
---|---|
gene PHO84, DNA and amino acid sequence determination and analysis, expression analysis | Saccharomyces cerevisiae |
gene PHT1;1 mapped to locus to chromosome V, expression analysis, overview | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of the s(V)-tolerant missense mutant pht1;1-3 results in decreased phosphate content and enhanced arsenic accumulation, the mutant accumulates double the arsenic found in wild-type plants, the As(V) tolerance phenotypes are enhanced in the pht1;1-3 expressor lines, pht1;1-3 exhibits a constitutive phosphate starvation response, overview | Arabidopsis thaliana |
additional information | strain PAM1 is transformed with PHO84pht1;1-3, a mutagenized version of the PHO84 cDNA, encoding a Gly-to-Glu mutation identical to the one present in the Arabidopsis thaliana pht1;1-3 allele. In the presence of 550 mM Pi, cells expressing PHO84pht1;1-3 exhibits more phosphatase activity than cells transformed with either PHO84 cDNA or the empty vector PAM1, the rate of Pi and As(V) transport in cells expressing PHO84pht1;1-3 is significantly lower than in the original PAM1, overview | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenic acid | As(V) represses the activation of genes specifically involved in Pi uptake, while inducing others transcriptionally regulated by As(V), suggesting that converse signaling pathways are involved in plant responses to As(V) and low phosphate availability, As(V) downregulates genes transcriptionally regulated by Pi starvation, being particularly efficient in the repression of the Pi/As(V) uptake system. The repression occurs conversely to the activation of As(V)-responsive genes, overview | Arabidopsis thaliana | |
arsenic acid | As(V) downregulates genes transcriptionally regulated by Pi starvation, being particularly efficient in the repression of the Pi/As(V) uptake system. The repression occurs conversely to the activation of As(V)-responsive genes, overview | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
membrane | - |
Arabidopsis thaliana | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | Saccharomyces cerevisiae | - |
ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | Arabidopsis thaliana | - |
ADP + phosphate + phosphate/in | - |
? | |
additional information | Arabidopsis thaliana | the exceptional toxicity of arsenate, As(V), is derived from its close chemical similarity to phosphate, which allows the metalloid to be easily incorporated into plant cells through the high-affinity Pi transport system | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
gene pht1;1 | - |
Saccharomyces cerevisiae | - |
strainPAM1, gene PHO84 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Arabidopsis thaliana | - |
root hair | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | - |
Saccharomyces cerevisiae | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | - |
Arabidopsis thaliana | ADP + phosphate + phosphate/in | - |
? | |
additional information | the exceptional toxicity of arsenate, As(V), is derived from its close chemical similarity to phosphate, which allows the metalloid to be easily incorporated into plant cells through the high-affinity Pi transport system | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
high-affinity Pi transporter | - |
Saccharomyces cerevisiae |
Pho84p | - |
Saccharomyces cerevisiae |
phosphate transporter | - |
Arabidopsis thaliana |
PHT1;1 | - |
Arabidopsis thaliana |
Pi transporter | - |
Saccharomyces cerevisiae |