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Literature summary for 7.3.2.1 extracted from

  • Gebhard Susann, G.S.; Tran Sieu , T.S.; Cook Gregory , C.G.
    The Phn system of Mycobacterium smegmatis: a second high-affinity ABC-transporter for phosphate (2006), Microbiology, 152, 3453-3465.
    View publication on PubMed
Search for more literature for 7.3.2.1

Activating Compound

Activating Compound Comment Organism Structure
additional information phosphate-limitation induces expression of phnD and pstS Mycolicibacterium smegmatis

Cloned(Commentary)

Cloned (Comment) Organism
pst and phn operons, the Phn system is encoded by a three-gene operon, DNA and amino acid sequence determinations and analysis, genomic organization, expression analyis of pstS and phnD, overview Mycolicibacterium smegmatis

Protein Variants

Protein Variants Comment Organism
additional information construction of deletion mutants, mutation of either pstS and phnD leads to impaired cell growth in minimal medium, the cells are only viable in high phosphate concentrated medium Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
arsenate strong inhibition Mycolicibacterium smegmatis
methylphosphonate competitive, partial inhibition of phosphate transport activity, overview Mycolicibacterium smegmatis
phosphite competitive, partial inhibition of phosphate transport activity, overview Mycolicibacterium smegmatis
phosphonate competitive, partial inhibition of phosphate transport activity, overview Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information phosphate transport kinetics in whole wild-type and mutant cells, overview Mycolicibacterium smegmatis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mycolicibacterium smegmatis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + phosphate/out Mycolicibacterium smegmatis the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out Mycolicibacterium smegmatis SG34 the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview ADP + phosphate + phosphate/in
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
 genes pstS and phnD
-
Mycolicibacterium smegmatis SG34
-
 genes pstS and phnD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphate/out the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview Mycolicibacterium smegmatis ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out
-
 Mycolicibacterium smegmatis ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview Mycolicibacterium smegmatis SG34 ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out
-
 Mycolicibacterium smegmatis SG34 ADP + phosphate + phosphate/in
-
 ?

Synonyms

Synonyms Comment Organism
high-affinity phosphate ABC-transporter
-
 Mycolicibacterium smegmatis
Phn
-
 Mycolicibacterium smegmatis
Pst
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mycolicibacterium smegmatis