Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D188K | no ATP hydrolyzing ability | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-p-fluorosulfonylbenzoyl adenosine | pH 7.4, 30 min at 30°C, 1-3 mM | Mycobacterium tuberculosis | |
guanidine hydrochloride | 5 min at 100°C, 8000 mM, significant reduction in the activity | Mycobacterium tuberculosis | |
Mn2+ | at room temperature | Mycobacterium tuberculosis | |
additional information | no inhibition with sodium azide, sodium orthovanadate and N-ethylmaleimide | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0239 | - |
ATP | at 37 °C, pH 7.5 | Escherichia coli | |
0.0715 | - |
ATP | at 37 °C, pH 7.5 | Mycobacterium tuberculosis | |
0.13 | - |
CTP | at 37 °C, pH 7.5 | Mycobacterium tuberculosis | |
0.211 | - |
GTP | at 37 °C, pH 7.5 | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inclusion body | - |
Escherichia coli | 16234 | - |
inclusion body | - |
Mycobacterium tuberculosis | 16234 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation with Ca2+, Mg2+ | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30500 | - |
PstB is the ATP-binding component, 2 * 30500, SDS-PAGE | Mycobacterium tuberculosis |
69300 | - |
gel filtration chromatography | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | Escherichia coli | ATP-consuming import of phosphate | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | Mycobacterium tuberculosis | ATP-consuming import of phosphate | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | Mycobacterium tuberculosis H37Rv | ATP-consuming import of phosphate | ADP + phosphate + phosphate/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | - |
Escherichia coli |
no modification | - |
Mycobacterium tuberculosis |
Purification (Comment) | Organism |
---|---|
by gel filtration chromatography using Superdex 200 column | Escherichia coli |
by gel permeation chromatography using Superdex 200 column | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | - |
Escherichia coli | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | - |
Mycobacterium tuberculosis | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | ATP-consuming import of phosphate | Escherichia coli | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | ATP-consuming import of phosphate | Mycobacterium tuberculosis | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | - |
Mycobacterium tuberculosis H37Rv | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | ATP-consuming import of phosphate | Mycobacterium tuberculosis H37Rv | ADP + phosphate + phosphate/in | - |
? | |
CTP + H2O + phosphate/out | - |
Mycobacterium tuberculosis | CDP + phosphate + phosphate/in | - |
? | |
CTP + H2O + phosphate/out | - |
Mycobacterium tuberculosis H37Rv | CDP + phosphate + phosphate/in | - |
? | |
GTP + H2O + phosphate/out | - |
Mycobacterium tuberculosis | GDP + phosphate + phosphate/in | - |
? | |
GTP + H2O + phosphate/out | - |
Mycobacterium tuberculosis H37Rv | GDP + phosphate + phosphate/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | PstB is the ATP-binding component, 2 * 30500, SDS-PAGE | Mycobacterium tuberculosis |
More | PstB is the ATP binding component | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
phosphate-specific transporter | - |
Escherichia coli |
phosphate-specific transporter | - |
Mycobacterium tuberculosis |
Pst | - |
Escherichia coli |
Pst | - |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 80 | pH 7.5, for 5 min, enzyme is heat-resistant | Mycobacterium tuberculosis |
37 | - |
pH 7.5, for 5 min, substantial loss in the enzyme activity at temperatures above 37°C | Escherichia coli |
80 | - |
pH 7.5 | Mycobacterium tuberculosis |
80 | - |
pH 7.5, between 30 and 60 min of heat, maximum loss of enzyme activity, 60% of initial value | Escherichia coli |
80 | - |
pH 7.5, incubating for 15 min, proteins are precipitated | Escherichia coli |
80 | - |
pH 7.5, thermal denaturation is evident within 5 min | Escherichia coli |
80 | - |
pH 7.5,incubating for 15 min, hardly shows any aggregation and for 1 h, loss 11% of its initial enzyme activity | Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
5'-p-fluorosulfonylbenzoyl adenosine | pH 7.4, at 30°C | Mycobacterium tuberculosis |