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Literature summary for 7.2.4.2 extracted from

  • Schmid, M.; Wild, M.R.; Dahinden, P.; Dimroth, P.
    Subunit gamma of the oxaloacetate decarboxylase Na(+) pump: interaction with other subunits/domains of the complex and binding site for the Zn(2+) metal ion (2002), Biochemistry, 41, 1285-1292.
    View publication on PubMed
Search for more literature for 7.2.4.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, expression of mutant enzymes and domains of the alpha subunit Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
D62A gamma subunit, Zn2+ content decreased to 35% of the wild type enzyme, roughly 30% of activity Klebsiella pneumoniae
D63A gamma subunit, almost no effect on activity Klebsiella pneumoniae
DELTAH76-P83 gamma subunit, unable to associate to the alpha subunit, no activity Klebsiella pneumoniae
DELTAH78-P83 gamma subunit, unable to associate to the alpha subunit, no activity Klebsiella pneumoniae
DELTAH82-P83 gamma subunit, Zn2+ content decreased to 5% of the wild type enzyme, very low activity Klebsiella pneumoniae
H76A gamma subunit, almost no effect on activity Klebsiella pneumoniae
H77A gamma subunit, Zn2+ content decreased to 10% of the wild type enzyme, very low activity Klebsiella pneumoniae
H78A gamma subunit, binding to alpha subunit is lost Klebsiella pneumoniae
H78A gamma subunit, unable to associate to the alpha subunit, no activity Klebsiella pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit Klebsiella pneumoniae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ binds to the gamma subunit, can not be removed with EDTA or 1,10-phenanthroline Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Oxaloacetate Klebsiella pneumoniae one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Pyruvate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Oxaloacetate
-
 Klebsiella pneumoniae Pyruvate + CO2
-
 ?
Oxaloacetate one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Klebsiella pneumoniae Pyruvate + CO2
-
 ?

Subunits

Subunits Comment Organism
trimer alpha, beta, gamma Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
biotin binds to the C-terminal domain of the alpha-subunit Klebsiella pneumoniae