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Literature summary for 7.2.4.2 extracted from

  • Jockel, P.; Schmid, M.; Steuber, J.; Dimroth, P.
    A molecular coupling mechanism for the oxaloacetate decarboxylase Na+ pump as inferred from mutational analysis (2000), Biochemistry, 39, 2307-2315.
    View publication on PubMed
Search for more literature for 7.2.4.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
D203N beta subunit, complete loss of activity Klebsiella pneumoniae
G337A beta subunit, complete loss of activity Klebsiella pneumoniae
G377A beta subunit, complete loss of activity Klebsiella pneumoniae
G380A beta subunit, about 30% of activity Klebsiella pneumoniae
additional information mutations in the beta subunit do not affect the ability to transfer the carboxyl-group to biotin, but reduce the decarboxylase activity Klebsiella pneumoniae
N373L beta subunit, almost complete loss of activity Klebsiella pneumoniae
N392L beta subunit, about 70% of activity Klebsiella pneumoniae
R389A beta subunit, about 10% of activity, significantly decreased Na+ binding Klebsiella pneumoniae
R389D beta subunit, almost complete loss of activity Klebsiella pneumoniae
R389K beta subunit, about 80% of activity, significantly decreased Na+ binding Klebsiella pneumoniae
R389L beta subunit, about 10% of activity, significantly decreased Na+ binding Klebsiella pneumoniae
S382A beta subunit, complete loss of activity Klebsiella pneumoniae
S382C beta subunit, complete loss of activity Klebsiella pneumoniae
S382D beta subunit, about 10% of activity Klebsiella pneumoniae
S382E beta subunit, complete loss of activity Klebsiella pneumoniae
S382N beta subunit, complete loss of activity Klebsiella pneumoniae
S382Q beta subunit, complete loss of activity Klebsiella pneumoniae
S382T beta subunit, about 10% of activity Klebsiella pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit Klebsiella pneumoniae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ two ions per enzyme required for maximum activity, cooperative binding Klebsiella pneumoniae
Zn2+ binds to the gamma subunit Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Oxaloacetate Klebsiella pneumoniae one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Pyruvate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein from Escherichia coli Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Oxaloacetate
-
 Klebsiella pneumoniae Pyruvate + CO2
-
 ?
Oxaloacetate one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Klebsiella pneumoniae Pyruvate + CO2
-
 ?

Subunits

Subunits Comment Organism
trimer alpha, beta, gamma Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 wild type enzyme Klebsiella pneumoniae
8.5
-
 pH-optima above 8.5 found for N373L, R389A, R389L mutant enzymes Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
biotin binds to the C-terminal domain of the alpha-subunit Klebsiella pneumoniae