Cloned (Comment) | Organism |
---|---|
all subunits, expressed in Escherichia coli, alpha-subunit strongly overexpressed from pT7-7 plasmid | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | - |
Klebsiella pneumoniae | |
Zn2+ | gamma-subunit, involved in carboxytransfer, 0.96 mol/mol enzyme | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate | Klebsiella pneumoniae | Na+ transport coupled to oxaloacetate decarboxylation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of reconstituted enzyme and isolated subunits | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | transmembrane transport of Na+ | Klebsiella pneumoniae | ? | - |
? | |
Oxaloacetate | - |
Klebsiella pneumoniae | Pyruvate + CO2 | - |
? | |
oxaloacetate | Na+ transport coupled to oxaloacetate decarboxylation | Klebsiella pneumoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | alpha-subunit also necessary for Na+ transport, spatial arrangement and subunit interactions | Klebsiella pneumoniae |