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Literature summary for 7.2.4.2 extracted from
- Preparation, characterization, and reconstitution of oxaloacetate decarboxylase from Klebsiella aerogenes, a sodium pump (1986), Methods Enzymol., 125, 530-540.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Oxomalonate | - |
Klebsiella aerogenes |  |
| Avidin | - |
Klebsiella aerogenes | |
| glyoxylate | - |
Klebsiella aerogenes | |
| L-malate | - |
Klebsiella aerogenes | |
| oxalate | competitive for oxaloacetate, Ki: 0.0038 mM | Klebsiella aerogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
oxaloacetate | - |
Klebsiella aerogenes | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Klebsiella aerogenes | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | Km: 1 mM | Klebsiella aerogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions | Klebsiella aerogenes |
| 34000 | - |
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions | Klebsiella aerogenes |
| 65000 | - |
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxaloacetate | Klebsiella aerogenes | decarboxylation energy used for active Na+ transport | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella aerogenes |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| oxaloacetate = pyruvate + CO2 | pyruvate + CO2 = oxaloacetae | Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Klebsiella aerogenes |
| 46.3 | - |
- |
Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | transmembrane transport of Na+ | Klebsiella aerogenes | ? | - |
? | |
| Oxaloacetate | - |
Klebsiella aerogenes | Pyruvate + CO2 | - |
? | |
| oxaloacetate | decarboxylation energy used for active Na+ transport | Klebsiella aerogenes | ? | - |
? | |
| Pyruvate + CO2 | - |
Klebsiella aerogenes | Oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions | Klebsiella aerogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
enzyme assay at | Klebsiella aerogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Klebsiella aerogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | about 1 mol bound per alpha-chain | Klebsiella aerogenes | |
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