BRENDA - Enzyme Database
show all sequences of 7.2.4.2

Preparation, characterization, and reconstitution of oxaloacetate decarboxylase from Klebsiella aerogenes, a sodium pump

Dimroth, P.; Methods Enzymol. 125, 530-540 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-Oxomalonate
-
Klebsiella aerogenes
Avidin
-
Klebsiella aerogenes
glyoxylate
-
Klebsiella aerogenes
L-malate
-
Klebsiella aerogenes
oxalate
competitive for oxaloacetate, Ki: 0.0038 mM
Klebsiella aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
oxaloacetate
-
Klebsiella aerogenes
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Klebsiella aerogenes
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Na+
Km: 1 mM
Klebsiella aerogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12000
-
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions
Klebsiella aerogenes
34000
-
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions
Klebsiella aerogenes
65000
-
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions
Klebsiella aerogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oxaloacetate
Klebsiella aerogenes
decarboxylation energy used for active Na+ transport
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella aerogenes
-
-
-
Purification (Commentary)
Commentary
Organism
-
Klebsiella aerogenes
Reaction
Reaction
Commentary
Organism
oxaloacetate = pyruvate + CO2
pyruvate + CO2 = oxaloacetae
Klebsiella aerogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Klebsiella aerogenes
46.3
-
-
Klebsiella aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
transmembrane transport of Na+
4260
Klebsiella aerogenes
?
-
-
-
-
Oxaloacetate
-
4260
Klebsiella aerogenes
Pyruvate + CO2
-
4260
Klebsiella aerogenes
-
oxaloacetate
decarboxylation energy used for active Na+ transport
4260
Klebsiella aerogenes
?
-
-
-
-
Pyruvate + CO2
-
4260
Klebsiella aerogenes
Oxaloacetate
-
4260
Klebsiella aerogenes
-
Subunits
Subunits
Commentary
Organism
trimer
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions
Klebsiella aerogenes
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
enzyme assay at
Klebsiella aerogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
-
Klebsiella aerogenes
Cofactor
Cofactor
Commentary
Organism
Structure
biotin
about 1 mol bound per alpha-chain
Klebsiella aerogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
biotin
about 1 mol bound per alpha-chain
Klebsiella aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-Oxomalonate
-
Klebsiella aerogenes
Avidin
-
Klebsiella aerogenes
glyoxylate
-
Klebsiella aerogenes
L-malate
-
Klebsiella aerogenes
oxalate
competitive for oxaloacetate, Ki: 0.0038 mM
Klebsiella aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
oxaloacetate
-
Klebsiella aerogenes
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Klebsiella aerogenes
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Na+
Km: 1 mM
Klebsiella aerogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12000
-
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions
Klebsiella aerogenes
34000
-
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions
Klebsiella aerogenes
65000
-
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions
Klebsiella aerogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oxaloacetate
Klebsiella aerogenes
decarboxylation energy used for active Na+ transport
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Klebsiella aerogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Klebsiella aerogenes
46.3
-
-
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
transmembrane transport of Na+
4260
Klebsiella aerogenes
?
-
-
-
-
Oxaloacetate
-
4260
Klebsiella aerogenes
Pyruvate + CO2
-
4260
Klebsiella aerogenes
-
oxaloacetate
decarboxylation energy used for active Na+ transport
4260
Klebsiella aerogenes
?
-
-
-
-
Pyruvate + CO2
-
4260
Klebsiella aerogenes
Oxaloacetate
-
4260
Klebsiella aerogenes
-
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
1 * 65000 + 1 * 34000 + 1 * 12000 alpha,beta,gamma, SDS-PAGE, subunits carry different functions
Klebsiella aerogenes
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
enzyme assay at
Klebsiella aerogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
-
Klebsiella aerogenes
Other publictions for EC 7.2.4.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749221
Inoue
Highly active and stable oxal ...
Vibrio cholerae
Protein Expr. Purif.
115
34-38
2015
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
1
1
-
-
-
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1
1
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-
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-
1
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1
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1
1
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-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650112
Schmid
Subunit gamma of the oxaloacet ...
Klebsiella pneumoniae
Biochemistry
41
1285-1292
2002
-
-
1
-
8
-
-
-
1
1
-
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
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1
1
-
8
-
-
-
-
-
1
1
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1
-
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-
1
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-
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649957
Jockel
A molecular coupling mechanism ...
Klebsiella pneumoniae
Biochemistry
39
2307-2315
2000
-
-
1
-
18
-
-
-
1
2
-
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
18
-
-
-
-
-
1
2
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
649974
Jockel
Essential role of tyrosine 229 ...
Klebsiella pneumoniae
Biochemistry
39
4320-4326
2000
-
-
1
-
20
-
-
-
1
2
-
1
-
1
-
-
1
-
-
-
2
-
2
1
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
20
-
-
-
-
-
1
2
-
1
-
-
-
1
-
-
2
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
649873
Jockel
Membrane topology of the beta- ...
Klebsiella pneumoniae
Biochemistry
38
13461-13472
1999
-
-
1
-
11
-
-
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
11
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4277
Di Bernardino
Cellular localisation by immun ...
Klebsiella pneumoniae
FEMS Microbiol. Lett.
136
31-37
1996
-
-
1
-
-
-
-
-
2
1
-
1
-
1
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3
1
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1
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2
1
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1
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-
-
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3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4274
Di Bernardino
Synthesis of the oxaloacetate ...
Klebsiella pneumoniae
Eur. J. Biochem.
231
790-801
1995
-
-
1
-
-
-
-
-
-
2
-
1
-
1
-
-
1
-
-
-
1
-
3
1
-
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-
-
-
-
-
-
-
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-
-
1
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-
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-
-
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2
-
1
-
-
-
1
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4282
Dimroth
On the mechanism of sodium ion ...
Klebsiella pneumoniae
Biochemistry
32
1734-1739
1993
-
-
-
-
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-
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1
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1
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1
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3
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1
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1
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3
-
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-
4280
Dimroth
The sodium ion pumping oxaloac ...
Klebsiella pneumoniae
FEBS Lett.
300
67-70
1992
-
-
-
-
-
-
4
-
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2
-
1
-
1
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3
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4
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2
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1
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3
-
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-
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-
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4278
Wifling
Isolation and characterization ...
Salmonella enterica subsp. enterica serovar Typhimurium
Arch. Microbiol.
152
584-588
1989
-
-
-
-
-
-
1
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1
3
2
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1
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1
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1
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4
1
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1
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1
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1
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1
3
2
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1
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1
-
4
1
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4258
Schwarz
The sodium ion translocating o ...
Klebsiella pneumoniae
J. Biol. Chem.
263
9640-9645
1988
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1
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1
3
1
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1
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1
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1
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1
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3
1
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4259
Dimroth
Dissociation of the sodium-ion ...
Klebsiella pneumoniae
Eur. J. Biochem.
175
175-180
1988
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1
1
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1
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3
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4281
Daekena
-
Oxaloacetate decarboxylase fro ...
Klebsiella pneumoniae
FEMS Microbiol. Lett.
55
35-40
1988
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1
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1
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1
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4260
Dimroth
Preparation, characterization, ...
Klebsiella aerogenes
Methods Enzymol.
125
530-540
1986
-
-
-
-
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5
1
1
1
3
1
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1
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1
1
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2
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4
1
1
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1
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1
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1
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5
-
1
1
1
3
1
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1
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2
-
4
1
1
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1
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4261
Dimroth
Kinetic analysis of the reacti ...
Klebsiella aerogenes
Eur. J. Biochem.
156
157-162
1986
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4
-
1
2
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1
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1
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3
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1
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4
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4
4
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1
2
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1
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3
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1
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-
4279
Dimroth
-
Carboxylation of pyruvate and ...
Klebsiella aerogenes
Biochemistry
23
5360-5366
1984
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-
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1
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1
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1
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1
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4
-
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1
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1
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4
-
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4262
Dimroth
Subunit composition of oxaloac ...
Klebsiella aerogenes
Eur. J. Biochem.
137
107-112
1983
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1
1
3
1
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1
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1
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1
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3
1
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-
-
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-
1
-
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-
-
-
-
1
-
-
-
-
-
-
-
1
1
3
1
-
-
-
1
-
-
1
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3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4264
Dimroth
Purification of the sodium tra ...
Klebsiella aerogenes
FEBS Lett.
141
59-62
1982
-
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-
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1
1
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1
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1
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1
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3
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1
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1
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1
1
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1
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1
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3
-
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4265
Dimroth
The generation of an electroch ...
Klebsiella aerogenes
Eur. J. Biochem.
121
443-449
1982
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1
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1
1
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2
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1
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4
-
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1
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1
1
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2
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4
-
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4266
Dimroth
The role of biotin and sodium ...
Klebsiella aerogenes
Eur. J. Biochem.
121
435-441
1982
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-
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1
1
1
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1
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1
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1
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3
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1
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1
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1
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1
1
1
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3
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1
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4267
Dimroth
Characterization of a membrane ...
Klebsiella aerogenes
Eur. J. Biochem.
115
353-358
1981
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1
6
1
1
1
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1
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1
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3
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1
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1
1
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1
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1
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6
1
1
1
1
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1
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3
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1
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