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Literature summary for 7.2.2.9 extracted from

  • Barry, A.N.; Otoikhian, A.; Bhatt, S.; Shinde, U.; Tsivkovskii, R.; Blackburn, N.J.; Lutsenko, S.
    The lumenal loop Met672-Pro707 of copper-transporting ATPase ATP7A binds metals and facilitates copper release from the intramembrane sites (2011), J. Biol. Chem., 286, 26585-26594.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
M672G/M674A/H676A/H677G/H682A/H683G the mutant shows decreased affinity for copper. The mutations within the His/Met-rich segment Met672-Pro707 (HM-loop) do not prevent the ability of the enzyme to form a phosphorylated intermediate during ATP hydrolysis but inhibit subsequent dephosphorylation, a step associated with copper release Homo sapiens
M687A/M692A the mutant shows decreased affinity for copper. The mutations within the His/Met-rich segment Met672-Pro707 (HM-loop) do not prevent the ability of the enzyme to form a phosphorylated intermediate during ATP hydrolysis but inhibit subsequent dephosphorylation, a step associated with copper release Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
copper the enzyme contains two Cu(I)-binding sites. Copper binding within the His-Met-loop stabilizes Cu(I) and protects it from oxidation, which may further aid the transfer of copper from ATP7A to acceptor proteins Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q04656
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
chitin resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cu2+/in
-
 Homo sapiens ADP + phosphate + Cu2+/out
-
 ?

Synonyms

Synonyms Comment Organism
ATP7A
-
 Homo sapiens
copper-transporting ATPase
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction inactivation of ATP7A results in the neurodegenerative disorder Menkes disease Homo sapiens