Literature summary for 7.2.2.9 extracted from

Banci, L.; Bertini, I.; Cantini, F.; Inagaki, S.; Migliardi, M.; Rosato, A.
The binding mode of ATP revealed by the solution structure of the N-domain of human ATP7A (2010), J. Biol. Chem., 285, 2537-2544.

Search for more literature for 7.2.2.9

Cloned(Commentary)

Cloned (Comment)	Organism
gene ATP7A, overexpression of His-tagged ATP7A or MNK in Escherichia coli strain BL21(DE3), overexpression of the soluble nucleotide-binding domain of MNK protein, comprising amino acids 1051-1231	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
solution structures of ATP-free and ATP-bound N-MNK	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + Cu2+/in	Homo sapiens	-	ADP + phosphate + Cu2+/out	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q04656	gene ATP7A	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged ATP7A or MNK from Escherichia coli strain BL21(DE3)	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Cu2+/in	-	Homo sapiens	ADP + phosphate + Cu2+/out	-	?
ATP + H2O + Cu2+/in	the ATP binding cavity contains mainly hydrophobic aliphatic residues, which are involved in van der Waals interactions with the adenine ring of ATP, and a Glu side chain, which forms a crucial hydrogen bond to the amino group of ATP, binding mode of ATP, detailed overview	Homo sapiens	ADP + phosphate + Cu2+/out	-	?

Subunits

Subunits	Comment	Organism
More	analysis of the solution NMR structures of the N-domain of ATP7A in the ATP-free and ATP-bound forms, The structures consist of a twisted antiparallel six-stranded beta-sheet flanked by two pairs of alpha-helices, overview. Structure comparisons to Archeoglobus fulgidus CopA, overview	Homo sapiens

Synonyms

Synonyms	Comment	Organism
ATP7A	-	Homo sapiens
copper(I)-transporting PIB-type ATPase	-	Homo sapiens
Menkes protein	-	Homo sapiens
MNK	-	Homo sapiens

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Release 2023.1 (January 2023)