Cloned (Comment) | Organism |
---|---|
expression in Spodoptera frugiperda Sf-9 cells | Homo sapiens |
infection of Sf9 cells with baculoviral constructs encoding wild-type ATP7B and a mutant variant unable to transport copper | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | CPA mutant analyzed, transmembrane transport function disabled by conversion of the transmembrane metal binding CPC motif to CPA | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cisplatin | DDP, shown to inhibit the transport of copper, pH 4.6 | Homo sapiens | |
Cu2+ | shown to inhibit the transport of cisplatin | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu2+/in | Homo sapiens | - |
ADP + phosphate + Cu2+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35670 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
ATP-dependent accumulation of cisplatin indicated, cisplatin shown to induce acyl-phosphorylation of ATP7B but at slower rate than copper, Vmax value for cisplatin shown to be nearly 28fold lower than that for copper | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu2+/in | - |
Homo sapiens | ADP + phosphate + Cu2+/out | - |
? | |
ATP + H2O + Cu2+/in | ATP7B shown to regulate cellular efflux of cisplatin (DDP), control of sensitivity to cytotoxic effects determined | Homo sapiens | ADP + phosphate + Cu2+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP7B | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
transport assay at | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.6 | 6.9 | ability of wild-type ATP7B to transport copper when exposed to 2 microM copper shown to be greater at pH 4.6 than at pH 6.9, ATP-dependent accumulation of cisplatin shown at 4.6 | Homo sapiens |