Cloned (Comment) | Organism |
---|---|
enzyme is expressed using pGEX-4T-2, subcloned into pGEX-6P-2 and expressed in Escherichia coli strain BL21 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu2+/in | Homo sapiens | the copper-induced conformational changes could play an important role in the function and regulation of the ATPase in vivo | ADP + phosphate + Cu2+/out | - |
? | |
ATP + H2O + Cu2+/in | Homo sapiens | the Wilson disease copper-transporting ATPase plays a critical role in the intracellular trafficking of copper. Mutations in this protein lead to the accumulation of a toxic level of copper in the liver, kidney, and brain followed by extensive tissue damage and death | ADP + phosphate + Cu2+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu2+/in | each binding site ligates copper in the +1 oxidation state using two cysteine side chains with distorted linear geometry, both secondary and tertiary changes take place upon copper binding | Homo sapiens | ADP + phosphate + Cu2+/out | - |
? | |
ATP + H2O + Cu2+/in | the copper-induced conformational changes could play an important role in the function and regulation of the ATPase in vivo | Homo sapiens | ADP + phosphate + Cu2+/out | - |
? | |
ATP + H2O + Cu2+/in | the Wilson disease copper-transporting ATPase plays a critical role in the intracellular trafficking of copper. Mutations in this protein lead to the accumulation of a toxic level of copper in the liver, kidney, and brain followed by extensive tissue damage and death | Homo sapiens | ADP + phosphate + Cu2+/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the amino-terminal domain of the enzyme contains six repeats of the copper binding motif GMTCXXC | Homo sapiens |