Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cupriavidus metallidurans |
Protein Variants | Comment | Organism |
---|---|---|
C476A | the mutant enzyme binds 0.61 mol equiv of Co2+. The mutant enzyme shows about 4%, about 24%, and about 30% of Cd2+-, Co2+-, and Zn2+-stimulated ATP hydrolysis activity, respectively, compared to the wild type enzyme | Cupriavidus metallidurans |
H807A | the mutant enzyme retains the ability to bind about 1 mol equiv of all metal ions (Co2+, Zn2+, and Cd2+). The mutation results in a strong decrease in Co2+- (about 17% of wild type) and Zn2+-stimulated (about 16% of wild type) ATP hydrolysis | Cupriavidus metallidurans |
M254A | the mutant enzyme binds 0.34 mol and 0.26 mol equiv of Cd2+ and Co2+, respectively. The mutation dramatically reduces Cd2+- and Co2+-stimulated ATPase activity (about 8% and about 32% of wild type activity, respectively), whereas Zn2+-stimulated ATPase activity remains virtually unchanged | Cupriavidus metallidurans |
S474A | the mutant enzyme binds 0.45 mol equiv of Co2+. The mutation modestly decreases Co2+-stimulated ATP hydrolysis (about 66% of wild type activity) whereas Cd2+- and Zn2+-stimulated ATP hydrolysis remain virtually unchanged | Cupriavidus metallidurans |
S474A/C476A | the mutant enzyme shows about 2%, about 16%, and about 20% of Cd2+-, Co2+-, and Zn2+-stimulated ATP hydrolysis activity, respectively, compared to the wild type enzyme | Cupriavidus metallidurans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+[side 1] | Cupriavidus metallidurans | highest activity | ADP + phosphate + Cd2+[side 2] | - |
? | |
ATP + H2O + Co2+[side 1] | Cupriavidus metallidurans | - |
ADP + phosphate + Co2+[side 2] | - |
? | |
ATP + H2O + Zn2+[side 1] | Cupriavidus metallidurans | lowest activity | ADP + phosphate + Zn2+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus metallidurans | Q1LAJ7 | formerly named Alcaligenes eutrophus and Ralstonia metallidurans | - |
Purification (Comment) | Organism |
---|---|
Ni-charged HiTrap column chromatography and Ni-NTA column chromatography | Cupriavidus metallidurans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
with Cd2+ as transport ion, at pH 7.5 and 30°C | Cupriavidus metallidurans |
0.06 | - |
with Co2+ as transport ion, at pH 7.5 and 30°C | Cupriavidus metallidurans |
0.2 | - |
with Cd2+ as transport ion, at pH 7.5 and 30°C | Cupriavidus metallidurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+[side 1] | highest activity | Cupriavidus metallidurans | ADP + phosphate + Cd2+[side 2] | - |
? | |
ATP + H2O + Co2+[side 1] | - |
Cupriavidus metallidurans | ADP + phosphate + Co2+[side 2] | - |
? | |
ATP + H2O + Zn2+[side 1] | lowest activity | Cupriavidus metallidurans | ADP + phosphate + Zn2+[side 2] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CzcP | - |
Cupriavidus metallidurans |