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Literature summary for 7.2.2.14 extracted from

  • Datiles, M.J.; Johnson, E.A.; McCarty, R.E.
    Inhibition of the ATPase activity of the catalytic portion of ATP synthases by cationic amphiphiles (2008), Biochim. Biophys. Acta, 1777, 362-368.
    View publication on PubMed
Search for more literature for 7.2.2.14

Activating Compound

Activating Compound Comment Organism Structure
cetyltrimethylammonium bromide i.e. CTAB, Mg2+-ATPase activity of ECF1 is very resistant to inhibition by cetyltrimethylammonium bromide, lower concentrations of cetyltrimethylammonium bromide stimulate Mg2+-ATPase activity of ECF1 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information Mg2+-ATPase activities of CF1 deficient in its inhibitory epsilon subunit, CF1-epsilon, are sensitive to inhibition by melittin and by cetyltrimethylammonium bromide. Sulfite-activated Mg2+-ATPase activity of CF1-epsilon is inhibited by cetyltrimethylammonium bromide Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
cetyltrimethylammonium bromide Mg2+-ATPase activity of ECF1 is very resistant to inhibition by cationic detergents melittin and cetyltrimethylammonium bromide, lower concentrations of cetyltrimethylammonium bromide stimulate Mg2+-ATPase activity of ECF1. MgATP protects, overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Mg2+/out Escherichia coli
-
 ADP + phosphate + Mg2+/in
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Mg2+/out
-
 Escherichia coli ADP + phosphate + Mg2+/in
-
 ?

Synonyms

Synonyms Comment Organism
ECF1
-
 Escherichia coli
Mg2+-ATPase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01 0.015 pH 8.0, 37°C, inhibitory epsilon subunit, CF1-epsilon Escherichia coli cetyltrimethylammonium bromide
0.025
-
 pH 8.0, 37°C, ECF1 Escherichia coli cetyltrimethylammonium bromide