Literature summary for 7.2.2.14 extracted from

Weghuber, J.;Dieterich, F.; Froschauer, E.M.; Svidova, S.; Schweyen, R.J.
Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae (2006), FEBS J., 273, 1198-1209.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant enzyme, the arginine rich motif of Mrs2p is not essential for the splicing of group II introns	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
D235R	loss-of-function mutation, site-directed mutagenesis, growth phenotype, overview	Saccharomyces cerevisiae
D244K	loss-of-function mutation, site-directed mutagenesis, growth phenotype, overview	Saccharomyces cerevisiae
M1301	gain-of-function mutation, random mutagenesis	Saccharomyces cerevisiae
additional information	gain-of-function mutations, obtained upon random mutagenesis, map to the conserved primary sequences blocking in the central part of Mrs2, while site-directed mutations in several conserved sequences reduce Mrs2p-mediated Mg2+ uptake and decrease group II intron splicing both resulting in reduced steady-state Mg2+ concentrations, overview	Saccharomyces cerevisiae
R173E	loss-of-function mutation, site-directed mutagenesis, growth phenotype, overview	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrial inner membrane	Mrs2p contains two adjacent transmembrane domains near the C terminus of Mrs2p one of which ends with a F/Y-G-M-N motif	Saccharomyces cerevisiae	5743	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + Mg2+/out	Saccharomyces cerevisiae	-	ADP + phosphate + Mg2+/in	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q02783	nuclear gene MRS2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Mg2+/out	-	Saccharomyces cerevisiae	ADP + phosphate + Mg2+/in	-	?

Subunits

Subunits	Comment	Organism
More	two coiled-coil domains and several conserved primary sequences block in the central part of Mrs2p	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
mitochondrial Mg2+ channel protein	-	Saccharomyces cerevisiae
More	the enzyme belongs to the Mrs2 protein family	Saccharomyces cerevisiae
Mrs2p	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)