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Literature summary for 7.2.2.13 extracted from

  • Laursen, M.; Gregersen, J.L.; Yatime, L.; Nissen, P.; Fedosova, N.U.
    Structures and characterization of digoxin- and bufalin-bound Na+,K+-ATPase compared with the ouabain-bound complex (2015), Proc. Natl. Acad. Sci. USA, 112, 1755-1760.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.13

Crystallization (Commentary)

Crystallization (Comment) Organism
E2P enzyme form in complex with inhibitors, hanging drop vapor diffusion method, using 17% (w/v) PEG 2000 monomethyl ether, 10% (v/v) glycerol, 220 mM MgCl2,100 mM KCl, 100 mM MES (titrated with KOH, pH 6.2), 5% (v/v) 2-methyl-2,4-pentanediol, and 6% (v/v) Jeffamine M-600 (titrated with KOH, pH 6.2) Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
bufalin
-
 Sus scrofa
digitoxigenin
-
 Sus scrofa
digoxin
-
 Sus scrofa
ouabagenin
-
 Sus scrofa
Ouabain
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Na+/in + K+/out Sus scrofa
-
 ADP + phosphate + Na+/out + K+/in
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa P05024
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
 at pH 7.0 and 37°C Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Na+/in + K+/out
-
 Sus scrofa ADP + phosphate + Na+/out + K+/in
-
 ?

Synonyms

Synonyms Comment Organism
Na+,K+-ATPase
-
 Sus scrofa