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Literature summary for 7.2.2.13 extracted from

  • Leone, F.A.; Lucena, M.N.; Rezende, L.A.; Garcon, D.P.; Pinto, M.R.; Mantelatto, F.L.; McNamara, J.C.
    A kinetic characterization of (Na+, K+)-ATPase activity in the gills of the pelagic seabob shrimp Xiphopenaeus kroyeri (Decapoda, Penaeidae) (2015), J. Membr. Biol., 248, 257-272.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
orthovanadate about 44% inhibition at 3 mM Xiphopenaeus kroyeri
Ouabain about 50% inhibition at 3 mM, ouabain inhibition increases about 80% in the presence of NH4+ with a 3fold lower KI value Xiphopenaeus kroyeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
ATP at pH 7.4 and 25°C Xiphopenaeus kroyeri

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Xiphopenaeus kroyeri
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulates activity Xiphopenaeus kroyeri
Mg2+ stimulates activity Xiphopenaeus kroyeri
additional information K+ plus NH4+ do not synergistically stimulate (Na+, K+)-ATPase activity Xiphopenaeus kroyeri
Na+ stimulates activity Xiphopenaeus kroyeri
NH4+ stimulates activity Xiphopenaeus kroyeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
x * 110000, alpha subunit, SDS-PAGE Xiphopenaeus kroyeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Na+/in + K+/out Xiphopenaeus kroyeri
-
ADP + phosphate + Na+/out + K+/in
-
?

Organism

Organism UniProt Comment Textmining
Xiphopenaeus kroyeri
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
gill
-
Xiphopenaeus kroyeri
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Na+/in + K+/out
-
Xiphopenaeus kroyeri ADP + phosphate + Na+/out + K+/in
-
?

Subunits

Subunits Comment Organism
? x * 110000, alpha subunit, SDS-PAGE Xiphopenaeus kroyeri

Synonyms

Synonyms Comment Organism
Na+, K+-ATPase
-
Xiphopenaeus kroyeri

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000157
-
orthovanadate at pH 7.4 and 25°C Xiphopenaeus kroyeri
0.084
-
Ouabain at pH 7.4 and 25°C Xiphopenaeus kroyeri