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Literature summary for 7.2.2.13 extracted from

  • Holm, R.; Einholm, A.P.; Andersen, J.P.; Vilsen, B.
    Rescue of Na+ affinity in aspartate 928 mutants of Na+,K+-ATPase by secondary mutation of glutamate 314 (2015), J. Biol. Chem., 290, 9801-9811.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in COS-1 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D928A the mutant exhibits a modest reduction of the apparent Na+ affinity to 5fold relative to the wild type enzyme Rattus norvegicus
D928E the mutant exhibits a modest reduction of the apparent Na+ affinity to 4fold relative to the wild type enzyme Rattus norvegicus
D928H the mutant exhibits a modest reduction of the apparent Na+ affinity to 7fold relative to the wild type enzyme Rattus norvegicus
D928L the mutant exhibits a modest reduction of the apparent Na+ affinity to 10fold relative to the wild type enzyme Rattus norvegicus
D928N the mutant exhibits a dramatic reduction of the apparent Na+ affinity to 78fold and slight 1.5fold increase of apparent K+ affinity relative to the wild type enzyme Rattus norvegicus
E314D the mutant exhibits an 3.2fold increase of the apparent Na+ affinity and 1.7fold increase of apparent K+ affinity relative to the wild type enzyme Rattus norvegicus
E314D/D928A the mutant exhibits an 1.2fold increase of the apparent Na+ affinity and slight 1.7fold decrease of apparent K+ affinity relative to the wild type enzyme Rattus norvegicus
E314D/D928N the mutant exhibits a modest 8.1fold decrease of the apparent Na+ affinity and slight 1.5fold increase of apparent K+ affinity relative to the wild type enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ouabain
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Na+/in + K+/out Rattus norvegicus
-
 ADP + phosphate + Na+/out + K+/in
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Na+/in + K+/out
-
 Rattus norvegicus ADP + phosphate + Na+/out + K+/in
-
 ?

Synonyms

Synonyms Comment Organism
Na+,K+-ATPase
-
 Rattus norvegicus