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Literature summary for 7.2.2.13 extracted from

  • Zhang, Y.R.; Yuan, Z.Y.
    Dopamine-mediated inhibition of renal Na+/K+-ATPase in HK-2 cells is reduced by ouabain (2010), Clin. Exp. Pharmacol. Physiol., 37, 613-618.
    View publication on PubMed
Search for more literature for 7.2.2.13

Activating Compound

Activating Compound Comment Organism Structure
Ouabain pretreatment of HK-2 cells with ouabain increases the Na+K+ATPase activity and inhibits the inhibitory effect of the D1 receptor on enzyme levels and activity, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
fenoldopam induces inhibition of Na+ ?K+-ATPase activity in HK-2 cells. Ouabain protects against the cyclic adenosine monophosphate accumulation and Na+ ?K+-ATPase inhibition induced by the D1 receptor agonist fenoldopam in HK-2 cells. Chronic ouabain treatment decreases the protein and mRNA expression levels of the D1 receptor and increases the basal phosphorylation of the D1 receptor in HK-2 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Na+/in + K+/out Homo sapiens
-
 ADP + phosphate + Na+/out + K+/in
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HK-2 cell proximal tubule cell line Homo sapiens
-
kidney
-
 Homo sapiens
-
proximal tubule
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.133
-
 enzyme activity in HK-2 cells in presence of fenoldopam, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.183
-
 enzyme activity in HK-2 cells, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.25
-
 enzyme activity in ouabain-pretreated HK-2 cells in presence of fenoldopam, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.267
-
 enzyme activity in ouabain-pretreated HK-2 cells, pH not specified in the publication, temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Na+/in + K+/out
-
 Homo sapiens ADP + phosphate + Na+/out + K+/in
-
 ?

Synonyms

Synonyms Comment Organism
Na+/K+-ATPase
-
 Homo sapiens
renal sodium-potassium pump
-
 Homo sapiens