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Literature summary for 7.2.2.13 extracted from

  • Takada, Y.; Matsuo, K.; Ogura, H.; Bai, L.; Toki, A.; Wang, L.; Ando, M.; Kataoka, T.
    Odoroside A and ouabain inhibit Na+/K+-ATPase and prevent NF-kappaB-inducible protein expression by blocking Na+-dependent amino acid transport (2009), Biochem. Pharmacol., 78, 1157-1166.
    View publication on PubMed
Search for more literature for 7.2.2.13

Inhibitors

Inhibitors Comment Organism Structure
additional information odoroside A and ouabain potently reduce NF-kappaB-inducible de novo protein synthesis, largely due to its ability to block Na+-dependent transport of amino acids across the plasma membrane, but not to interfering with the translation machinery Homo sapiens
odoroside A i.e. 3beta-O-(beta-D-diginosyl)-14-hydroxy-5beta,14beta-card-20(22)-enolide, isolated from the stems and twigs of Nerum oleander, inhibits the enzyme's ATPase activity Homo sapiens
Ouabain ouabain does not affect TNF-alpha-induced mRNA expression but inhibits TNF-alpha-induced protein expression Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Na+/in + K+/out Homo sapiens
-
 ADP + phosphate + Na+/out + K+/in
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
 Homo sapiens
-
lung carcinoma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Na+/in + K+/out
-
 Homo sapiens ADP + phosphate + Na+/out + K+/in
-
 ?
ATP + H2O + Na+/in + Rb+/out measurement of enzyme activity by ouabain-sensitive 86Rb+ uptake Homo sapiens ADP + phosphate + Na+/out + Rb+/in
-
 ?

Synonyms

Synonyms Comment Organism
Na+/K+-ATPase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0012
-
 pH 7.0, 37°C Homo sapiens odoroside A
0.0016
-
 pH 7.0, 37°C Homo sapiens Ouabain

General Information

General Information Comment Organism
additional information odoroside A and ouabain potently reduce NF-kappaB-inducible de novo protein synthesis, largely due to its ability to block Na+-dependent transport of amino acids across the plasma membrane, but not to interfering with the translation machinery Homo sapiens