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Literature summary for 7.2.2.13 extracted from

  • Capendeguy, O.; Horisberger, J.D.
    The role of the third extracellular loop of the Na+,K+-ATPase alpha subunit in a luminal gating mechanism (2005), J. Physiol., 565, 207-218.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.13

Cloned(Commentary)

Cloned (Comment) Organism
in pSD5 vector, expression of proteins in Xenopus laevis oocytes Rhinella marina

Protein Variants

Protein Variants Comment Organism
C111S control Rhinella marina
G803C the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. Rhinella marina
L802C the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. Rhinella marina
P801C the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. Rhinella marina
T804C the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. Rhinella marina
V805C the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. Rhinella marina

Inhibitors

Inhibitors Comment Organism Structure
MTSET [2-(trimethylammonium)ethyl]methanethiosulphonate bromide, inhibits mutants G803C, V805C Rhinella marina
Omeprazole inhibit mutants G803C, T804C, V805C Rhinella marina
Ouabain g-strophanthin Rhinella marina

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rhinella marina 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 glycosylated beta subunit Rhinella marina
110000
-
 alpha subunit Rhinella marina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphate + Na+/out Rhinella marina
-
 ATP + H2O + Na+/in
-
 ?
ATP + H2O + K+/in Rhinella marina
-
 ADP + phosphate + K+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Rhinella marina
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate + Na+/out
-
 Rhinella marina ATP + H2O + Na+/in
-
 ?
ATP + H2O + K+/in
-
 Rhinella marina ADP + phosphate + K+/out
-
 ?

Subunits

Subunits Comment Organism
dimer heterodimer, alpha and beta subunit Rhinella marina

Synonyms

Synonyms Comment Organism
Na+,K+-ATPase
-
 Rhinella marina