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Literature summary for 7.2.2.13 extracted from
- Nitrophenylphosphate as a tool to characterize gill Na(+), K(+)-ATPase activity in hyperregulating Crustacea (2001), Comp. Biochem. Physiol. A, 130, 665-676.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | competitive inhibitor of K+-phosphatase activity | Macrobrachium olfersii |  |
| Ethacrynic acid | - |
Macrobrachium olfersii | |
| Na+ | 25% inhibition at 80 mmol/l | Macrobrachium olfersii | |
| orthovanadate | - |
Macrobrachium olfersii | |
| Ouabain | - |
Macrobrachium olfersii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 1.72 mmol/l with p-nitrophenyl phosphate as substrate, 0.165 mmol/l with ATP as substrate | Macrobrachium olfersii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Macrobrachium olfersii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Macrobrachium olfersii | |
| Mg2+ | - |
Macrobrachium olfersii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Macrobrachium olfersii | - |
freshwater shrimp | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Macrobrachium olfersii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 259.1 | - |
with p-nitrophenolphosphate as substrate | Macrobrachium olfersii |
| 686.1 | - |
with ATP as substrate | Macrobrachium olfersii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, microsomal fraction, after 2 months no loss in activity | Macrobrachium olfersii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Macrobrachium olfersii | ADP + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Macrobrachium olfersii | p-nitrophenol + phosphate | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.05 mmol/l with ATP as inhibitor, 0.762 mmol/l with ouabain as inhibitor, 0.00025 mmol/l with o-vanadate as inhibitor | Macrobrachium olfersii |
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