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Literature summary for 7.2.2.12 extracted from

  • Leitenmaier, B.; Witt, A.; Witzke, A.; Stemke, A.; Meyer-Klaucke, W.; Kroneck, P.M.; Kuepper, H.
    Biochemical and biophysical characterisation yields insights into the mechanism of a Cd/Zn transporting ATPase purified from the hyperaccumulator plant Thlaspi caerulescens (2011), Biochim. Biophys. Acta, 1808, 2591-2599.
    View publication on PubMed
Search for more literature for 7.2.2.12

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates at 0.00003 mM, the level of activation does not change much until a concentration of 0.001 mM Noccaea caerulescens
Cu2+ activates at 0.00003 mM Noccaea caerulescens
Zn2+ activates at 0.00003 mM, the level of activation does not change much until a concentration of 0.0003 mM Noccaea caerulescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Noccaea caerulescens

Organism

Organism UniProt Comment Textmining
Noccaea caerulescens Q70LF4 ecotype Ganges
-

Purification (Commentary)

Purification (Comment) Organism
Ni-IDA metal affinity column chromatography, gel filtration Noccaea caerulescens

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Noccaea caerulescens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Zn2+/in
-
 Noccaea caerulescens ADP + phosphate + Zn2+/out
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Noccaea caerulescens

Synonyms

Synonyms Comment Organism
Cd/Zn transporting ATPase
-
 Noccaea caerulescens
HMA4
-
 Noccaea caerulescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
 Noccaea caerulescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
300
-
 ATP in the presence of Zn2+ or Cd2+, in 0.6 M Tris, 2 M NaCl and 0.1 M MgCl2, pH 8.0, at 30°C Noccaea caerulescens