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Literature summary for 7.2.2.12 extracted from

  • Wong, C.K.; Cobbett, C.S.
    HMA P-type ATPases are the major mechanism for root-to-shoot Cd translocation in Arabidopsis thaliana (2009), New Phytol., 181, 71-78.
    View publication on PubMed
Search for more literature for 7.2.2.12

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type HMA2 and mutant derivatives in transgenic hma2,hma4, Zn-deficient plants from the HMA2 promoter, quantitative realtime PCR expression analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information expression of mutant derivatives, with and without a C-terminal GFP tag, from the HMA2 promoter in transgenic hma2,hma4, Zn-deficient plants. The deletion mutant lacking the C-terminal 244 amino acids rescues most of the hma2,hma4 Zn-deficiency phenotypes with the exception of embryo or seed development. Root-to-shoot Cd translocation is fully rescued. The GFP-tagged derivative is partially mislocalized in the root pericycle cells in which it is expressed. Deletion derivatives lacking the C-terminal 121 and 21 amino acids rescue all phenotypes and localize normally. N-terminal domain mutants localize normally but fail to complement the hma2,hma4 phenotypes, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Arabidopsis thaliana 5886
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Zn2+/in Arabidopsis thaliana the N-terminal domain of HMA2 is essential for function in planta while the C-terminal domain, although not essential for function, may contain a signal important for the subcellular localization of the protein ADP + phosphate + Zn2+/out
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 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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 ecotype Col-0
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Source Tissue

Source Tissue Comment Organism Textmining
pericycle
-
 Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Zn2+/in the N-terminal domain of HMA2 is essential for function in planta while the C-terminal domain, although not essential for function, may contain a signal important for the subcellular localization of the protein Arabidopsis thaliana ADP + phosphate + Zn2+/out
-
 ?
ATP + H2O + Zn2+/in HMA2 has N- and C-terminal domains that can bind Zn ions with high affinity Arabidopsis thaliana ADP + phosphate + Zn2+/out
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 ?

Synonyms

Synonyms Comment Organism
HMA2
-
 Arabidopsis thaliana
additional information the enzyme is a member of the P1B subfamily of the ATPases Arabidopsis thaliana
Zn/Cd-transporting ATPase
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 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana