Any feedback?
Literature summary for 7.2.2.12 extracted from
- A novel regulatory metal binding domain is present in the C terminus of Arabidopsis Zn2+-ATPase HMA2 (2006), J. Biol. Chem., 281, 33881-33891.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Arabidopsis thaliana HMA2 and truncated forms of the protein are prepared using the bacterial expression vector pPRIBA1 and the yeast expression vector pYES2/CT | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C-MBD | HMA2 C-terminal metal binding domain | Arabidopsis thaliana |
| DELTAC-HMA2 | mutant, lacking the 244 C-terminal amino acids | Arabidopsis thaliana |
| DELTANC-HMA2 | mutant, lacking the N-terminal first 75 amino acids and the 244 C-terminal amino acids | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Arabidopsis thaliana | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Zn2+/in | Arabidopsis thaliana | - |
ADP + phosphate + Zn2+/out | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| membranes from yeast are prepared, purification of the HMA2 C-terminal metal binding domain is performed using a Strep-Tactin Superflow column | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Zn2+/in | - |
Arabidopsis thaliana | ADP + phosphate + Zn2+/out | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HMA2 | - |
Arabidopsis thaliana |
| Zn2+-ATPase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
ATPase activity assay | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
ATPase activity assay | Arabidopsis thaliana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
