Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Arabidopsis thaliana | |
Zn2+ | - |
Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
the yeast expression vector pYES2/CT carrying Arabidopsis thaliana HMA2 containing a C-terminal Strep-tag is prepared, the HMA2 N-terminal metal binding domain is cloned into the pPRIBA1 vector for expression in Escherichia coli BL21DE3pLysS cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C17A | mutant, 36% decrease in Vmax | Arabidopsis thaliana |
C18A | mutant, 34% decrease in Vmax | Arabidopsis thaliana |
DELTAN-HMA2 | mutant, lacking the N-terminal first 75 amino acids, 56% decrease in Vmax | Arabidopsis thaliana |
E21A | mutant, 43% decrease in Vmax | Arabidopsis thaliana |
E21C | mutant, 60% decrease in Vmax | Arabidopsis thaliana |
S20A | mutant, shows Vmax similar to that of wild-type HMA2 | Arabidopsis thaliana |
S20C | mutant, shows Vmax similar to that of wild-type HMA2 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Arabidopsis thaliana | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | Arabidopsis thaliana | - |
ADP + phosphate + Cd2+/out | - |
? | |
ATP + H2O + Zn2+/in | Arabidopsis thaliana | - |
ADP + phosphate + Zn2+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of the HMA2 N-terminal metal binding domain is performed using Strep-tag affinity chromatography, membranes from HMA2 expressing yeasts are prepared | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-80°C, 100 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM Tris(2-carboxyethyl)phosphine hydrochoride | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | - |
Arabidopsis thaliana | ADP + phosphate + Cd2+/out | - |
? | |
ATP + H2O + Zn2+/in | - |
Arabidopsis thaliana | ADP + phosphate + Zn2+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HMA2 | - |
Arabidopsis thaliana |
Zn(2+)-ATPase | - |
Arabidopsis thaliana |
Zn2+ transporting P1B-type ATPase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
ATPase activity assay | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
ATPase activity assay | Arabidopsis thaliana |