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Literature summary for 7.2.2.12 extracted from

  • Eren, E.; Gonzalez-Guerrero, M.; Kaufman, B.M.; Argueello, J.M.
    Novel Zn2+ coordination by the regulatory N-terminus metal binding domain of Arabidopsis thaliana Zn(2+)-ATPase HMA2 (2007), Biochemistry, 46, 7754-7764.
    View publication on PubMed
Search for more literature for 7.2.2.12

Activating Compound

Activating Compound Comment Organism Structure
Cd2+
-
 Arabidopsis thaliana
Zn2+
-
 Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
the yeast expression vector pYES2/CT carrying Arabidopsis thaliana HMA2 containing a C-terminal Strep-tag is prepared, the HMA2 N-terminal metal binding domain is cloned into the pPRIBA1 vector for expression in Escherichia coli BL21DE3pLysS cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C17A mutant, 36% decrease in Vmax Arabidopsis thaliana
C18A mutant, 34% decrease in Vmax Arabidopsis thaliana
DELTAN-HMA2 mutant, lacking the N-terminal first 75 amino acids, 56% decrease in Vmax Arabidopsis thaliana
E21A mutant, 43% decrease in Vmax Arabidopsis thaliana
E21C mutant, 60% decrease in Vmax Arabidopsis thaliana
S20A mutant, shows Vmax similar to that of wild-type HMA2 Arabidopsis thaliana
S20C mutant, shows Vmax similar to that of wild-type HMA2 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Arabidopsis thaliana 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cd2+/in Arabidopsis thaliana
-
 ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Zn2+/in Arabidopsis thaliana
-
 ADP + phosphate + Zn2+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of the HMA2 N-terminal metal binding domain is performed using Strep-tag affinity chromatography, membranes from HMA2 expressing yeasts are prepared Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-80°C, 100 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM Tris(2-carboxyethyl)phosphine hydrochoride Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cd2+/in
-
 Arabidopsis thaliana ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Zn2+/in
-
 Arabidopsis thaliana ADP + phosphate + Zn2+/out
-
 ?

Synonyms

Synonyms Comment Organism
HMA2
-
 Arabidopsis thaliana
Zn(2+)-ATPase
-
 Arabidopsis thaliana
Zn2+ transporting P1B-type ATPase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 ATPase activity assay Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 ATPase activity assay Arabidopsis thaliana