Cloned (Comment) | Organism |
---|---|
into the pBAD/Myc-Hic C or pBAD/Myc-His A vector for expression in the Escherichia coli strain LMG194(zntA::cat) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C392A | mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding | Escherichia coli |
C392H | mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding | Escherichia coli |
C392H/C394H | mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding | Escherichia coli |
C392S | mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding | Escherichia coli |
C392S/C394S | mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding | Escherichia coli |
C394A | mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding | Escherichia coli |
C394S | mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding | Escherichia coli |
P393A | mutant, shows no activity and can not bind any metal ion at the transmembrane site | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
Ni2+ | wild-type ZntA | Escherichia coli | |
0.0031 | - |
Cd2+ | wild-type ZntA | Escherichia coli | |
0.0034 | - |
Cu2+ | wild-type ZntA | Escherichia coli | |
0.004 | - |
Co2+ | wild-type ZntA | Escherichia coli | |
0.0051 | - |
Zn2+ | wild-type ZntA | Escherichia coli | |
0.0061 | - |
Pb2+ | wild-type ZntA | Escherichia coli | |
0.007 | - |
Co2+ | mutant C392H | Escherichia coli | |
0.007 | - |
Zn2+ | mutant C392S/C394S | Escherichia coli | |
0.007 | - |
Cu2+ | mutant C394S | Escherichia coli | |
0.007 | - |
Co2+ | mutant C394S | Escherichia coli | |
0.007 | - |
Ni2+ | mutant C394S | Escherichia coli | |
0.008 | - |
Co2+ | mutant C394H | Escherichia coli | |
0.009 | - |
Zn2+ | mutant C392S | Escherichia coli | |
0.009 | - |
Ni2+ | mutant C394H | Escherichia coli | |
0.009 | - |
Cd2+ | mutant C394S | Escherichia coli | |
0.01 | - |
Zn2+ | mutant C392H | Escherichia coli | |
0.01 | - |
Cu2+ | mutant C392H/C394H | Escherichia coli | |
0.01 | - |
Cd2+ | mutant C392H/C394H | Escherichia coli | |
0.01 | - |
Cu2+ | mutant C392S | Escherichia coli | |
0.01 | - |
Cd2+ | mutant C394H | Escherichia coli | |
0.011 | - |
Cd2+ | mutant C392S | Escherichia coli | |
0.012 | - |
Cd2+ | mutant C392H | Escherichia coli | |
0.012 | - |
Co2+ | mutant C392S | Escherichia coli | |
0.012 | - |
Zn2+ | mutant C394H | Escherichia coli | |
0.013 | - |
Cu2+ | mutant C392H | Escherichia coli | |
0.013 | - |
Ni2+ | mutant C392H | Escherichia coli | |
0.013 | - |
Zn2+ | mutant C392H/C394H | Escherichia coli | |
0.013 | - |
Ni2+ | mutant C392H/C394H | Escherichia coli | |
0.013 | - |
Ni2+ | mutant C392S | Escherichia coli | |
0.013 | - |
Cd2+ | mutant C392S/C394S | Escherichia coli | |
0.013 | - |
Cu2+ | mutant C394H | Escherichia coli | |
0.014 | - |
Co2+ | mutant C392H/C394H | Escherichia coli | |
0.014 | - |
Zn2+ | mutant C394S | Escherichia coli | |
0.018 | - |
Co2+ | mutant C392S/C394S | Escherichia coli | |
0.019 | - |
Cu2+ | mutant C392S/C394S | Escherichia coli | |
0.066 | - |
Co2+ | + thiolate, mutant C392H/C394H | Escherichia coli | |
0.075 | - |
Co2+ | + thiolate, wild-type ZntA | Escherichia coli | |
0.08 | - |
Ni2+ | + thiolate, mutant C392H | Escherichia coli | |
0.08 | - |
Ni2+ | + thiolate, mutant C392S | Escherichia coli | |
0.083 | - |
Zn2+ | + thiolate, mutant C392H/C394H | Escherichia coli | |
0.083 | - |
Ni2+ | + thiolate, mutant C394H | Escherichia coli | |
0.085 | - |
Cu2+ | + thiolate, wild-type ZntA | Escherichia coli | |
0.091 | - |
Cd2+ | + thiolate, mutant C392H/C394H | Escherichia coli | |
0.092 | - |
Co2+ | + thiolate, mutant C394H | Escherichia coli | |
0.096 | - |
Co2+ | + thiolate, mutant C392S/C394S | Escherichia coli | |
0.099 | - |
Zn2+ | + thiolate, mutant C392S/C394S | Escherichia coli | |
0.104 | - |
Co2+ | + thiolate, mutant C394S | Escherichia coli | |
0.105 | - |
Cu2+ | + thiolate, mutant C392H/C394H | Escherichia coli | |
0.105 | - |
Zn2+ | + thiolate, wild-type ZntA | Escherichia coli | |
0.11 | - |
Cd2+ | + thiolate, mutant C394H | Escherichia coli | |
0.114 | - |
Cd2+ | + thiolate, mutant C392H | Escherichia coli | |
0.116 | - |
Ni2+ | + thiolate, mutant C392H/C394H | Escherichia coli | |
0.117 | - |
Ni2+ | + thiolate, mutant C394S | Escherichia coli | |
0.123 | - |
Cd2+ | + thiolate, wild-type ZntA | Escherichia coli | |
0.124 | - |
Cu2+ | + thiolate, mutant C392S | Escherichia coli | |
0.126 | - |
Co2+ | + thiolate, mutant C392S | Escherichia coli | |
0.127 | - |
Cu2+ | + thiolate, mutant C392S/C394S | Escherichia coli | |
0.131 | - |
Zn2+ | + thiolate, mutant C392H | Escherichia coli | |
0.131 | - |
Zn2+ | + thiolate, mutant C394H | Escherichia coli | |
0.133 | - |
Co2+ | + thiolate, mutant C392H | Escherichia coli | |
0.139 | - |
Cd2+ | + thiolate, mutant C392S/C394S | Escherichia coli | |
0.141 | - |
Zn2+ | + thiolate, mutant C392S | Escherichia coli | |
0.145 | - |
Cu2+ | + thiolate, mutant C394S | Escherichia coli | |
0.148 | - |
Cd2+ | + thiolate, mutant C392S | Escherichia coli | |
0.15 | - |
Pb2+ | + thiolate, wild-type ZntA | Escherichia coli | |
0.152 | - |
Cd2+ | + thiolate, mutant C394S | Escherichia coli | |
0.158 | - |
Cu2+ | + thiolate, mutant C392H | Escherichia coli | |
0.169 | - |
Ni2+ | + thiolate, wild-type ZntA | Escherichia coli | |
0.171 | - |
Cu2+ | + thiolate, mutant C394H | Escherichia coli | |
0.182 | - |
Zn2+ | + thiolate, mutant C394S | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | Escherichia coli | - |
ADP + phosphate + Cd2+/out | - |
? | |
ATP + H2O + Co2+/in | Escherichia coli | - |
ADP + phosphate + Co2+/out | - |
? | |
ATP + H2O + Cu2+/in | Escherichia coli | - |
ADP + phosphate + Cu2+/out | - |
? | |
ATP + H2O + Ni2+/in | Escherichia coli | - |
ADP + phosphate + Ni2+/out | - |
? | |
ATP + H2O + Pb2+/in | Escherichia coli | - |
ADP + phosphate + Pb2+/out | - |
? | |
ATP + H2O + Zn2+/in | Escherichia coli | - |
ADP + phosphate + Zn2+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Talon-affinity column | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | - |
Escherichia coli | ADP + phosphate + Cd2+/out | - |
? | |
ATP + H2O + Co2+/in | - |
Escherichia coli | ADP + phosphate + Co2+/out | - |
? | |
ATP + H2O + Cu2+/in | - |
Escherichia coli | ADP + phosphate + Cu2+/out | - |
? | |
ATP + H2O + Ni2+/in | - |
Escherichia coli | ADP + phosphate + Ni2+/out | - |
? | |
ATP + H2O + Pb2+/in | - |
Escherichia coli | ADP + phosphate + Pb2+/out | - |
? | |
ATP + H2O + Zn2+/in | - |
Escherichia coli | ADP + phosphate + Zn2+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ZntA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
ATPase activity assay | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
ATPase activity assay | Escherichia coli |