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Literature summary for 7.2.2.12 extracted from

  • Liu, J.; Dutta, S.J.; Stemmler, A.J.; Mitra, B.
    Metal-binding affinity of the transmembrane site in ZntA: implications for metal selectivity (2006), Biochemistry, 45, 763-772.
    View publication on PubMed
Search for more literature for 7.2.2.12

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes C59A/C62A and C392A/C394A Escherichia coli

Protein Variants

Protein Variants Comment Organism
C392A/C394A mutant enzyme binds metal ions only at the N-terminal site Escherichia coli
C59A/C62A mutant enzyme in which the N-terminal metal-binding site is disabled by site-specific mutagenesis, can bind only one metal ion Escherichia coli
additional information DELTA-ZntA, in which the N-terminal domain is deleted can bind only one metal ion Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Cs2+ ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli. Wild-type ZntA binds two metal ions with high affinity, one in the N-terminal domain and another in the transmembrane domain Escherichia coli
Pb2+ ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli. Wild-type ZntA binds two metal ions with high affinity, one in the N-terminal domain and another in the transmembrane domain Escherichia coli
Zn2+ ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli. Wild-type ZntA binds two metal ions with high affinity, one in the N-terminal domain and another in the transmembrane domain. Wild-type ZntA binds two metal ions with high affinity, one in the N-terminal domain and another in the transmembrane domain Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cd2+/in Escherichia coli ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Pb2+/in Escherichia coli ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli ADP + phosphate + Pb2+/out
-
 ?
ATP + H2O + Zn2+/in Escherichia coli ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli ADP + phosphate + Zn2+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzymes C59A/C62A and C392A/C394A Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cd2+/in ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli Escherichia coli ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Pb2+/in ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli Escherichia coli ADP + phosphate + Pb2+/out
-
 ?
ATP + H2O + Zn2+/in ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli Escherichia coli ADP + phosphate + Zn2+/out
-
 ?

Synonyms

Synonyms Comment Organism
ZntA
-
 Escherichia coli