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Literature summary for 7.2.2.12 extracted from

  • Mitra, B.; Sharma, R.
    The cysteine-rich amino-terminal domain of ZntA, a Pb(II)/Zn(II)/Cd(II)-translocating ATPase from Escherichia coli, is not essential for its function (2001), Biochemistry, 40, 7694-7699.
    View publication on PubMed
Search for more literature for 7.2.2.12

Activating Compound

Activating Compound Comment Organism Structure
L-cysteine activates the enzyme and mutant lacking 100 residues Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information Construction of a mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC. The activity of the mutant is similar to the wild-type. The Km for ATP is unchanged. The function of the amino-terminal domain may be to increase the overall catalytic rate by increasing the rate of binding of specific metal ions to the transporter. Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 Cd2+ pH 7.0, 37°C, mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC Escherichia coli
0.005
-
 Pb2+ pH 7.0, 37°C, enzyme Escherichia coli
0.0055
-
 Cd2+ pH 7.0, 37°C, enzyme Escherichia coli
0.009
-
 Zn2+ pH 7.0, 37°C, mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC Escherichia coli
0.01
-
 Zn2+ pH 7.0, 37°C, enzyme Escherichia coli
0.012
-
 Pb2+ pH 7.0, 37°C, mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC Escherichia coli
0.064
-
 ATP pH 7.0, 37°C, mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC Escherichia coli
0.075
-
 ATP pH 7.0, 37°C, enzyme Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 the mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC, SDS-PAGE Escherichia coli
80000
-
 wild-type, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cd2+/in
-
 Escherichia coli ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Pb2+/in
-
 Escherichia coli ADP + phosphate + Pb2+/out
-
 ?
ATP + H2O + Zn2+/in
-
 Escherichia coli ADP + phosphate + Zn2+/out
-
 ?