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Literature summary for 7.2.2.10 extracted from

  • Dode, L.; Andersen, J.P.; Vanoevelen, J.; Raeymaekers, L.; Missiaen, L.; Vilsen, B.; Wuytack, F.
    Dissection of the functional differences between human secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1 and 2 isoenzymes by steady-state and transient kinetic analyses (2006), J. Biol. Chem., 281, 3182-3189.
    View publication on PubMed
Search for more literature for 7.2.2.10

Cloned(Commentary)

Cloned (Comment) Organism
genes ATP2C1 and ATP2C2, transient overexpression of isozymes SPCA1 and SPCA2 in HEK293 cell microsomal membranes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
vanadate inhibits the phosphorylation reaction Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information isoenzymes SPCA 1 and 2, steady-state and transient kinetic analyses of Ca2+ transport and of phosphorylation and dephosphorylation reactions, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ SPCA1d shows a 2.5fold higher affinity for Ca2+ compared to SPCA2 Homo sapiens
K+ slightly stimulates the dephosphorylation reaction Homo sapiens
Mg2+ required Homo sapiens
Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Ca2+/cis Homo sapiens Ca2+-dependent ATPase and ATP-dependent Ca2+ transport activities ADP + phosphate + Ca2+/trans
-
 ?
ATP + H2O + Mn2+/cis Homo sapiens Mn2+-dependent ATPase and ATP-dependent Mn2+ transport activities ADP + phosphate + Mn2+/trans
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 genes ATP2C1 and ATP2C2, isoenzymes SPCA 1 and 2
-

Purification (Commentary)

Purification (Comment) Organism
partially recombinant isozymes SPCA1 and SPCA2 in HEK293 cell microsomal membranes by differential centrifugation Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + Ca2+[side 1] = ADP + phosphate + Ca2+[side 2] kinetic mechanism of isozymes SPCA1 and SPCA2, phosphorylated intermediate, overview Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information isozyme SPCA1 is ubiquitously expressed, while SPCA2 is limited to some tissues Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Ca2+/cis Ca2+-dependent ATPase and ATP-dependent Ca2+ transport activities Homo sapiens ADP + phosphate + Ca2+/trans
-
 ?
ATP + H2O + Mn2+/cis Mn2+-dependent ATPase and ATP-dependent Mn2+ transport activities Homo sapiens ADP + phosphate + Mn2+/trans
-
 ?
additional information dissection of the functional differences between secretory pathway Ca2+/Mn2+-ATPase isoenzymes SPCA 1 and 2 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
secretory pathway Ca2+/Mn2+-ATPase
-
 Homo sapiens
SPCA
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 isozyme SPCA2, about 20% of maximal activity at pH 6.0, and about 60% at pH 8.5, isozyme SPCA1d, about 35% of maximal activity at pH 6.0, and about 60% at pH 8.5 Homo sapiens