Cloned (Comment) | Organism |
---|---|
genes ntpE and ntpF encoding the two subunits of the enzyme, expression of both subunits and the N-terminal soluble domain of NtpI as His-tagged proteins in Escherichia coli strain BL21(DE3) | Enterococcus hirae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuolar membrane | the enzyme is orientated to the cytoplasmic side, structural model of V-ATPase, overview | Enterococcus hirae | 5774 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Na+/in | Enterococcus hirae | - |
ADP + phosphate + Na+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus hirae | - |
genes ntpE and ntpF | - |
Purification (Comment) | Organism |
---|---|
separate purification of His-tagged NtpE and NtpF, and His-tagged N-terminal hydrophilic domain of NtpI by nickel affinity and ion exchange chromatography, and gel filtration | Enterococcus hirae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Na+/in | - |
Enterococcus hirae | ADP + phosphate + Na+/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the peripheral stalk of Na+-translocating V-ATPase of Enterococcus hirae is composed of NtpE and NtpF subunits together with the N-terminal hydrophilic domain of NtpI, structural model of V-ATPase, overview. Subunit interaction analysis by pulldown assay and surface plasmon resonance, detailed overview | Enterococcus hirae |
Synonyms | Comment | Organism |
---|---|---|
Na+-translocating V-ATPase | - |
Enterococcus hirae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Enterococcus hirae |