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Literature summary for 7.2.2.1 extracted from
- Purification of ATP synthase from Acetobacterium woodii and identification as a Na+-translocating F1F0-type enzyme (1994), Eur. J. Biochem., 223, 275-283.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfite | stimulates activity | Acetobacterium woodii |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-(N,N-hexamethylene)-amiloride | - |
Acetobacterium woodii | |
| 5-(N-ethyl-N-isopropyl)-amiloride | - |
Acetobacterium woodii | |
| ADP | - |
Acetobacterium woodii | |
| azide | - |
Acetobacterium woodii | |
| Ca2+ | - |
Acetobacterium woodii | |
| Cr2+ | - |
Acetobacterium woodii | |
| Cu2+ | - |
Acetobacterium woodii | |
| N,N'-dicyclohexylcarbodiimide | reacts with the 4800 Da subunit | Acetobacterium woodii | |
| N-10-benzylamiloride | - |
Acetobacterium woodii | |
| Ni2+ | - |
Acetobacterium woodii | |
| NO3- | - |
Acetobacterium woodii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Acetobacterium woodii | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity is dependent on MgATP, at pH 8.5 optimal Mg2+ concentration is 2 mM | Acetobacterium woodii | |
| Na+ | the enzyme has Na+-translocating activity | Acetobacterium woodii | |
| Na+ | strictly dependent on Na+, Km: 0.4 mM | Acetobacterium woodii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE | Acetobacterium woodii |
| 19000 | - |
x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE | Acetobacterium woodii |
| 35000 | - |
x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE | Acetobacterium woodii |
| 52000 | - |
x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE | Acetobacterium woodii |
| 57000 | - |
x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE | Acetobacterium woodii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacterium woodii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetobacterium woodii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Acetobacterium woodii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Acetobacterium woodii | ADP + phosphate | - |
? | |
| CTP + H2O | at 9% of the activity with ATP | Acetobacterium woodii | CDP + H2O | - |
? | |
| GTP + H2O | hydrolyzed at 31% of the activity with ATP | Acetobacterium woodii | GDP + phosphate | - |
? | |
| ITP + H2O | hydrolyzed at 39% of the activity with ATP | Acetobacterium woodii | ? | - |
? | |
| UTP + H2O | at 4% of the activity with ATP | Acetobacterium woodii | UDP + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE | Acetobacterium woodii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| F1FO-ATPase | - |
Acetobacterium woodii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Acetobacterium woodii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable for longer periods | Acetobacterium woodii |
| 50 | 55 | stable for 5-10 min | Acetobacterium woodii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | in presence of 0.1 mM Na+ | Acetobacterium woodii |
| 7 | 9 | in presence of 5 mM Na+ | Acetobacterium woodii |
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