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Literature summary for 7.2.1.3 extracted from

  • Wyman, S.; Simpson, R.J.; McKie, A.T.; Sharp, P.A.
    Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro (2008), FEBS Lett., 582, 1901-1906.
    View publication on PubMed
Search for more literature for 7.2.1.3

Activating Compound

Activating Compound Comment Organism Structure
dehydroascorbate preloading cells with dehydroascorbate greatly increases both ferric and cupric reductase activities of Dcytb Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
Dcytb-EGFP is expressed in tetracycline-off Madin-Darby canine kidney cells Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0152
-
 Cu(II)-nitrilotriacetic acid[side 2] in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 22°C Mus musculus
0.0231
-
 Cu(II)-nitrilotriacetic acid[side 2] in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 37°C Mus musculus
0.074
-
 Fe(III)-nitrilotriacetic acid[side 2] in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 37°C Mus musculus
0.0921
-
 Fe(III)-nitrilotriacetic acid[side 2] in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 22°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Mus musculus 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ascorbate[side 1] + Fe(III)[side 2] Mus musculus
-
 monodehydroascorbate[side 1] + Fe(II)[side 2]
-
 ?
cupric-histidine[side 1] + ? Mus musculus
-
 ?
-
 ?
ferric citrate[side 1] + ? Mus musculus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
duodenum
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ascorbate[side 1] + Fe(III)[side 2]
-
 Mus musculus monodehydroascorbate[side 1] + Fe(II)[side 2]
-
 ?
bathocuprionedisulfonate[side 1] + Cu(II)-nitrilotriacetic acid[side 2]
-
 Mus musculus Cu(I)-bathocuproinedisulfonate
-
 ?
cupric-histidine[side 1] + ?
-
 Mus musculus ?
-
 ?
ferric citrate[side 1] + ?
-
 Mus musculus ?
-
 ?
ferrozine[side 1] + Fe(III)-nitrilotriacetic acid[side 2]
-
 Mus musculus Fe(II)-ferrozine
-
 ?
additional information Dcytb has the capacity to reduce both iron and copper complexes Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
Cybrd1
-
 Mus musculus
DCytb
-
 Mus musculus
duodenal cytochrome b
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
ascorbate dependent on Mus musculus

Expression

Organism Comment Expression
Mus musculus expression of Dcytb-EGFP is knocked down by addition of 20 ng/ml doxycycline down

General Information

General Information Comment Organism
physiological function Dcytb plays a physiological role in both iron and copper uptake, through divalent metal transporter1 and copper transporter 1, respectively Mus musculus