Cloned (Comment) | Organism |
---|---|
nqr operon encoding all subunits A-F, genetic structure, recombinant expression of His6-tagged subunit NqrC in Escherichia coli, coexpression of flavin transferase ApbE is required for Na+-NQR enzyme activity | Vibrio harveyi |
nqr operon encoding all subunits A-F, genetic structure, recombinant expression of His6-tagged subunit NqrC in Escherichia coli, coexpression of flavin transferase ApbE is required for Na+-NQR enzyme activity | Klebsiella pneumoniae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Vibrio harveyi | 16020 | - |
membrane | - |
Klebsiella pneumoniae | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + ubiquinone + n Na+/in | Vibrio harveyi | - |
NAD+ + ubiquinol + n Na+/out | - |
r | |
NADH + H+ + ubiquinone + n Na+/in | Klebsiella pneumoniae | - |
NAD+ + ubiquinol + n Na+/out | - |
r | |
NADH + H+ + ubiquinone + n Na+/in | Vibrio harveyi R3 | - |
NAD+ + ubiquinol + n Na+/out | - |
r | |
NADH + H+ + ubiquinone + n Na+/in | Klebsiella pneumoniae 204 | - |
NAD+ + ubiquinol + n Na+/out | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae 204 | - |
- |
- |
Vibrio harveyi | - |
- |
- |
Vibrio harveyi R3 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + ubiquinone + n Na+/in | - |
Vibrio harveyi | NAD+ + ubiquinol + n Na+/out | - |
r | |
NADH + H+ + ubiquinone + n Na+/in | - |
Klebsiella pneumoniae | NAD+ + ubiquinol + n Na+/out | - |
r | |
NADH + H+ + ubiquinone + n Na+/in | - |
Vibrio harveyi R3 | NAD+ + ubiquinol + n Na+/out | - |
r | |
NADH + H+ + ubiquinone + n Na+/in | - |
Klebsiella pneumoniae 204 | NAD+ + ubiquinol + n Na+/out | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | Na+-NQR consists of six subunits (NqrA-F) encoded by six genes of the nqr operon | Vibrio harveyi |
hexamer | Na+-NQR consists of six subunits (NqrA-F) encoded by six genes of the nqr operon | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
Na+-NQR | - |
Vibrio harveyi |
Na+-NQR | - |
Klebsiella pneumoniae |
Na+-translocating NADH:quinone oxidoreductase | - |
Vibrio harveyi |
Na+-translocating NADH:quinone oxidoreductase | - |
Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains one noncovalently bound FAD molecule in subunit NqrF. The enzyme shows dependence on covalently bound flavin. ApbE is a modifying enzyme involved in the maturation of flavoproteins, which catalyzes Mg2+-dependent FMN transfer from FAD to Thr residues of flavoproteins in vitro and in vivo, ApbE is the only protein factor required for NqrC flavinylation. Flavinylation o f NqrC is Mg2+-dependent and proceeds with FAD but not FMN | Klebsiella pneumoniae | |
FAD | Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains one noncovalently bound FAD molecule in subunit NqrF. The enzyme shows dependence on covalently bound flavin. ApbE is a modifying enzyme involved in the maturation of flavoproteins, which catalyzes Mg2+-dependent FMN transfer from FAD to Thr residues of flavoproteins in vitro and in vivo, it catalyzes the flavinylation of truncated Vibrio harveyi NqrCsubunit of enzyme Na+-NQR at Thr229, ApbE is the only protein factor required for NqrC flavinylation. Flavinylation o fNqrC isMg2-dependent and proceeds with FAD but not FMN | Vibrio harveyi | |
FMN | Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains two FMN residues as redox-active prosthetic groups attached by a phosphoester bond to threonine residues in subunits NqrB and NqrC. The enzyme shows dependence on covalently bound flavin | Vibrio harveyi | |
FMN | Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains two FMN residues as redox-active prosthetic groups attached by a phosphoester bond to threonine residues in subunits NqrB and NqrC. The enzyme shows dependence on covalently bound flavin | Klebsiella pneumoniae | |
NAD+ | - |
Vibrio harveyi | |
NAD+ | - |
Klebsiella pneumoniae | |
NADH | - |
Vibrio harveyi | |
NADH | - |
Klebsiella pneumoniae | |
riboflavin | the enzyme contains one noncovalently bound riboflavin molecule in subunit NqrB | Vibrio harveyi | |
riboflavin | the enzyme contains one noncovalently bound riboflavin molecule in subunit NqrB | Klebsiella pneumoniae | |
[2Fe-2S]-center | the enzyme contains one [2Fe-2S] cluster in subunit NqrF | Vibrio harveyi | |
[2Fe-2S]-center | the enzyme contains one [2Fe-2S] cluster in subunit NqrF | Klebsiella pneumoniae |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of the apbE gene in Klebsiella pneumoniae, wherein the nqr operon and apbE are well separated in the chromosome, results in a complete loss of the quinone reductase activity of Na+-NQR | Klebsiella pneumoniae |
additional information | the enzyme shows dependence on covalently bound flavin | Vibrio harveyi |
additional information | the enzyme shows dependence on covalently bound flavin | Klebsiella pneumoniae |