Cloned (Comment) | Organism |
---|---|
wild-type and mutant forms of the Na+-NQR from Vibrio cholerae as recombinant proteins containing a His6-tag at the N-terminus of subunit NqrA in an enzyme-deficient mutant strain DELTAnqr of Vibrio cholerae | Vibrio cholerae serotype O1 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | preparation of membranes containing subcomplexes of Na+-NQR, NqrBCDEF, NqrACDEF, NqrABDEF, NqrABCEF, and NqrABCF, by expressing mutant forms of the Na+-NQR in Vibrio cholerae enzyme-deficient mutant strain DELTAnqr | Vibrio cholerae serotype O1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | specific inhibition | Vibrio cholerae serotype O1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Vibrio cholerae serotype O1 | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
213000 | - |
about, His6-tagged Na+-NQR complex, sequence calculation | Vibrio cholerae serotype O1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + ubiquinone + n Na+/in | Vibrio cholerae serotype O1 | - |
NAD+ + ubiquinol + n Na+/out | - |
? | |
NADH + H+ + ubiquinone + n Na+/in | Vibrio cholerae serotype O1 ATCC 39541 | - |
NAD+ + ubiquinol + n Na+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | A5F5X1 and A5F5X0 and A5F5Y7 and A5F5Y6 and A5F5Y3 and A5F5Y4 | A5F5X1: subunit NqrA, A5F5X0: subunit NqrB, A5F5Y7: subunit NqrC, A5F5Y6: subunit NqrD; A5F5Y3: subunit NqrE, A5F5Y4: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon. | - |
Vibrio cholerae serotype O1 ATCC 39541 | A5F5X1 and A5F5X0 and A5F5Y7 and A5F5Y6 and A5F5Y3 and A5F5Y4 | A5F5X1: subunit NqrA, A5F5X0: subunit NqrB, A5F5Y7: subunit NqrC, A5F5Y6: subunit NqrD; A5F5Y3: subunit NqrE, A5F5Y4: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon. | - |
Purification (Comment) | Organism |
---|---|
His6-tagged wild-type and mutant forms of the Na+-NQR from Vibrio cholerae mutant strain DELTAnqr by nickel affinity chromatography and gel filtration | Vibrio cholerae serotype O1 |
Renatured (Comment) | Organism |
---|---|
reconstitution of purified recombinant enzyme with riboflavin, enzyme is mixed with 0.1 mM riboflavin from a1 mM riboflavin stock solution in N,N-dimethylformamide, 10 min, 25°C | Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + ubiquinone + n Na+/in | - |
Vibrio cholerae serotype O1 | NAD+ + ubiquinol + n Na+/out | - |
? | |
NADH + H+ + ubiquinone + n Na+/in | - |
Vibrio cholerae serotype O1 ATCC 39541 | NAD+ + ubiquinol + n Na+/out | - |
? | |
NADH + H+ + ubiquinone-1 + n Na+/in | ubiquinone-1 acts as substrate for the oxidation of NADH by the holo-Na+-NQR coupled to Na+ translocation. Ubiquinoone-1 also accepts electrons in a non-physiological reaction catalyzed by isolated NqrF or subcomplexes containing the NqrF subunit. Both the coupled and the non-physiological modes of NADH oxidation with Q1 require FAD as cofactor | Vibrio cholerae serotype O1 | NAD+ + ubiquinol-1 + n Na+/out | - |
? | |
NADH + H+ + ubiquinone-1 + n Na+/in | ubiquinone-1 acts as substrate for the oxidation of NADH by the holo-Na+-NQR coupled to Na+ translocation. Ubiquinoone-1 also accepts electrons in a non-physiological reaction catalyzed by isolated NqrF or subcomplexes containing the NqrF subunit. Both the coupled and the non-physiological modes of NADH oxidation with Q1 require FAD as cofactor | Vibrio cholerae serotype O1 ATCC 39541 | NAD+ + ubiquinol-1 + n Na+/out | - |
? | |
NADH + H+ + ubiquinone-8 + n Na+/in | - |
Vibrio cholerae serotype O1 | NAD+ + ubiquinol-8 + n Na+/out | - |
? | |
NADH + H+ + ubiquinone-8 + n Na+/in | - |
Vibrio cholerae serotype O1 ATCC 39541 | NAD+ + ubiquinol-8 + n Na+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Na+-NQR | - |
Vibrio cholerae serotype O1 |
Na+-translocating NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
sodium ion-translocating NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Vibrio cholerae serotype O1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | noncovalently bound FAD | Vibrio cholerae serotype O1 | |
FMN | covalently bound FMN | Vibrio cholerae serotype O1 | |
NADH | - |
Vibrio cholerae serotype O1 | |
riboflavin | noncovalently bound riboflavin in purified Na+-NQR is not liberated from FMN but represents an intrinsic component of the Na+-NQR complex | Vibrio cholerae serotype O1 |
General Information | Comment | Organism |
---|---|---|
physiological function | the sodium ion-translocating NADH:quinone oxidoreductase from the human pathogen Vibrio cholerae is a respiratory membrane protein complex that couples the oxidation of NADHto the transport of Na+ across the bacterial membrane | Vibrio cholerae serotype O1 |