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Literature summary for 7.2.1.1 extracted from

  • Juarez, O.; Morgan, J.E.; Nilges, M.J.; Barquera, B.
    Energy transducing redox steps of the Na+-pumping NADH:quinone oxidoreductase from Vibrio cholerae (2010), Proc. Natl. Acad. Sci. USA, 107, 12505-12510.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.1.1

Protein Variants

Protein Variants Comment Organism
C76A mutant of subunit NqrF which lacks the 2Fe-2S-center cofactor and shows a 90% decrease in menadione reductase activity Vibrio cholerae serotype O1
D346A the redox reaction in this mutant is significantly inhibited and the Na+ sensitivity of CoQ reduction is completely abolished, while the apparent affinity for Na+ is essentially unchanged. In D346A, electron transfer between FMN from subunit NqrB and riboflavin is inhibited Vibrio cholerae serotype O1
D397A the reduction of subunit NqrB mutant D397A by NADH is very similar to the reduction of wild type enzyme but does not show sensitivity to Na+, and the rate of the stable neutral riboflavin radical to riboflavin-H2 is decreased 3fold. In NqrB-D397A, the reaction takes place in two steps: rapid oxidation of riboflavin and the two FMNs, followed by oxidation of FAD, at a 16fold lower rate Vibrio cholerae serotype O1
T225Y the mutant lacks FMN from subunit NqrC and is unable to produce an electrochemical potential under either partial or multiple turnover conditions Vibrio cholerae serotype O1
T236Y the mutant lacks FMN from subunit NqrB and is unable to produce an electrochemical potential under either partial or multiple turnover conditions Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + ubiquinone + n Na+/in Vibrio cholerae serotype O1
-
 NAD+ + ubiquinol + n Na+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + ubiquinone + n Na+/in
-
 Vibrio cholerae serotype O1 NAD+ + ubiquinol + n Na+/out
-
 ?

Synonyms

Synonyms Comment Organism
Na+-NQR
-
 Vibrio cholerae serotype O1
Na+-pumping NADH:quinone oxidoreductase
-
 Vibrio cholerae serotype O1
sodium pumping NADH:quinone oxidoreductase
-
 Vibrio cholerae serotype O1

Cofactor

Cofactor Comment Organism Structure
FAD contains one noncovalently bound FAD Vibrio cholerae serotype O1
FMN contains two FMNs covalently bound to the NqrB and NqrC subunits Vibrio cholerae serotype O1
NADH
-
 Vibrio cholerae serotype O1
riboflavin contains one noncovalently bound riboflavin Vibrio cholerae serotype O1
[2Fe-2S]-center contains one 2Fe-2S-center Vibrio cholerae serotype O1

General Information

General Information Comment Organism
metabolism Na+-NQR couples the free energy of electron transfer reactions to electrogenic pumping of sodium across the cell membrane. Na+ uptake by Na+-NQR is a nonelectrogenic process that occurs upon electron transfer from the 2Fe-2S-center to FMN of subunit NqrC, while the electrogenic transport of Na+ across the membrane, and likely release into solution, occurs upon electron transfer from FMN of subunit NqrB to riboflavin Vibrio cholerae serotype O1