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Literature summary for 7.2.1.1 extracted from

  • Casutt, M.S.; Wendelspiess, S.; Steuber, J.; Fritz, G.
    Crystallization of the Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae (2010), Acta Crystallogr. Sect. F, 66, 1677-1679.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
hexahistidine-tagged Na+-NQR is expressed in Vibrio cholerae strain DELTAnqr lacking expression of the NQR complex Vibrio cholerae serotype O1

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 40 mM KSCN, 21.0% PEG 2000 MME, 100 mM Tris acetate pH 8.5 and 8% (v/v) 1-propanol, cofactors such as FAD, riboflavin or NADH are not added during crystallization Vibrio cholerae serotype O1

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Vibrio cholerae serotype O1 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + ubiquinone + n Na+/in Vibrio cholerae serotype O1
-
NAD+ + ubiquinol + n Na+/out
-
?

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superdex 200 gel filtration Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + ubiquinone + n Na+/in
-
Vibrio cholerae serotype O1 NAD+ + ubiquinol + n Na+/out
-
?

Subunits

Subunits Comment Organism
heterohexamer Na+-NQR is a heterohexameric complex consisting of six different protein subunits (NqrA-NqrF) Vibrio cholerae serotype O1

Synonyms

Synonyms Comment Organism
Na+-NQR
-
Vibrio cholerae serotype O1
Na+-translocating NADH:quinone oxidoreductase
-
Vibrio cholerae serotype O1
sodium-translocating NADH:quinone oxidoreductase
-
Vibrio cholerae serotype O1

Cofactor

Cofactor Comment Organism Structure
FAD noncovalently bound Vibrio cholerae serotype O1
FMN two covalently bound FMNs Vibrio cholerae serotype O1
NADH
-
Vibrio cholerae serotype O1
riboflavin noncovalently bound Vibrio cholerae serotype O1
ubiquinone-8
-
Vibrio cholerae serotype O1
[2Fe-2S]-center
-
Vibrio cholerae serotype O1