Cloned (Comment) | Organism |
---|---|
hexahistidine-tagged Na+-NQR is expressed in Vibrio cholerae strain DELTAnqr lacking expression of the NQR complex | Vibrio cholerae serotype O1 |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 40 mM KSCN, 21.0% PEG 2000 MME, 100 mM Tris acetate pH 8.5 and 8% (v/v) 1-propanol, cofactors such as FAD, riboflavin or NADH are not added during crystallization | Vibrio cholerae serotype O1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Vibrio cholerae serotype O1 | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + ubiquinone + n Na+/in | Vibrio cholerae serotype O1 | - |
NAD+ + ubiquinol + n Na+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 200 gel filtration | Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + ubiquinone + n Na+/in | - |
Vibrio cholerae serotype O1 | NAD+ + ubiquinol + n Na+/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterohexamer | Na+-NQR is a heterohexameric complex consisting of six different protein subunits (NqrA-NqrF) | Vibrio cholerae serotype O1 |
Synonyms | Comment | Organism |
---|---|---|
Na+-NQR | - |
Vibrio cholerae serotype O1 |
Na+-translocating NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
sodium-translocating NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | noncovalently bound | Vibrio cholerae serotype O1 | |
FMN | two covalently bound FMNs | Vibrio cholerae serotype O1 | |
NADH | - |
Vibrio cholerae serotype O1 | |
riboflavin | noncovalently bound | Vibrio cholerae serotype O1 | |
ubiquinone-8 | - |
Vibrio cholerae serotype O1 | |
[2Fe-2S]-center | - |
Vibrio cholerae serotype O1 |