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Literature summary for 7.1.3.1 extracted from

  • White, P.J.; Marshall, J.; Smith, J.A.
    Substrate kinetics of the tonoplast H+-translocating inorganic pyrophosphatase and its activation by free Mg (1990), Plant Physiol., 93, 1063-1070 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
H+[side 1] at pH 8.0 and 38°C Kalanchoe daigremontiana
0.017
-
diphosphate at pH 8.0 and 38°C Kalanchoe daigremontiana

Localization

Localization Comment Organism GeneOntology No. Textmining
tonoplast
-
Kalanchoe daigremontiana
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ enzyme activity is maximal in the presence of K+ (K50 approximately 3 mM) Kalanchoe daigremontiana
Mg2+ the enzyme has a requirement for a Mg/diphosphate complex as substrate and free Mg2+ for activation. Half-maximal enzyme activity occurs at free 1.1 mM Mg2+ Kalanchoe daigremontiana
Na+ half-maximal activity at 12 mM Na+ Kalanchoe daigremontiana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O + H+[side 1] Kalanchoe daigremontiana
-
2 phosphate + H+[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Kalanchoe daigremontiana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Kalanchoe daigremontiana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O + H+[side 1]
-
Kalanchoe daigremontiana 2 phosphate + H+[side 2]
-
?

Synonyms

Synonyms Comment Organism
tonoplast H+-PPiase
-
Kalanchoe daigremontiana
tonoplast H+-translocating inorganic pyrophosphatase
-
Kalanchoe daigremontiana

General Information

General Information Comment Organism
physiological function the ancillary enzyme maintains the proton-motive force across the vacuolar membrane when the activity of the tonoplast H+-ATPase is restricted by substrate availability Kalanchoe daigremontiana