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Literature summary for 7.1.3.1 extracted from

  • Lemercier, G.; Espiau, B.; Ruiz, F.A.; Vieira, M.; Luo, S.; Baltz, T.; Docampo, R.; Bakalara, N.
    A pyrophosphatase regulating polyphosphate metabolism in acidocalcisomes is essential for Trypanosoma brucei virulence in mice (2004), J. Biol. Chem., 279, 3420-3425.
    View publication on PubMed
Search for more literature for 7.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ competitive Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 Polyphosphate in the presence of Zn2+, pH 7.5, 25°C Trypanosoma brucei
0.019
-
 diphosphate in the presence of Mg2+, pH 7.5, 25°C Trypanosoma brucei
0.373
-
 tripolyphosphate in the presence of Zn2+, pH 7.5, 25°C Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
acidocalcisome soluble Trypanosoma brucei 20022
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ CaCl2 at 0.1 mM favors the formation of large complexes Trypanosoma brucei
Mg2+ required Trypanosoma brucei
Zn2+ required for the hydrolysis of tripolyphosphate and polyphosphate Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47300
-
 calculated from predicted amino acid sequence Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Trypanosoma brucei important for energy metabolism, provides energy for biosynthetic reactions, essential for growth of bloodstream forms in mammalian host 2 phosphate
-
 ?
polyphosphate + H2O Trypanosoma brucei polyphosphate of 28 residues, hydrolyzed only in the presence of Zn2+, central role in the regulation of polyphosphate metabolism, supports recovery from hypoosmotic stress ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q7Z029
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Trypanosoma brucei 2 phosphate
-
 ?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions, essential for growth of bloodstream forms in mammalian host Trypanosoma brucei 2 phosphate
-
 ?
polyphosphate + H2O polyphosphate of 28 residues, hydrolyzed only in the presence of Zn2+, central role in the regulation of polyphosphate metabolism, supports recovery from hypoosmotic stress Trypanosoma brucei ?
-
 ?
polyphosphate + H2O polyphosphate of 28 residues, poor substrate, hydrolyzed only in the presence of Zn2+ Trypanosoma brucei ?
-
 ?
tripolyphosphate + H2O hydrolyzed only in the presence of Zn2+ Trypanosoma brucei ?
-
 ?

Synonyms

Synonyms Comment Organism
acidocalcisomal pyrophosphatase
-
 Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
 Polyphosphate in the presence of Zn2+, pH 7.5, 25°C Trypanosoma brucei
52
-
 tripolyphosphate in the presence of Zn2+, pH 7.5, 25°C Trypanosoma brucei
56
-
 diphosphate in the presence of Zn2+, pH 7.5, 25°C Trypanosoma brucei
385
-
 diphosphate in the presence of Mg2+, pH 7.5, 25°C Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 in the presence of Zn2+ Trypanosoma brucei
7.5
-
 in the presence of Mg2+ Trypanosoma brucei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.014
-
 Ca2+ hydrolysis of diphosphate, pH 7.5, 25°C, no inhibition of tripolyphosphate and polyphosphate hydrolysis Trypanosoma brucei

pI Value

Organism Comment pI Value Maximum pI Value
Trypanosoma brucei calculated from predicted amino acid sequence
-
 6