Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | 3.5fold increase at 50 mM | Vigna radiata | |
additional information | no activation with NaCl at 50 mM | Vigna radiata |
Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae BJ5458 | Vigna radiata |
Protein Variants | Comment | Organism |
---|---|---|
C304R | complete loss of activity, no proton pumping | Vigna radiata |
D279A | complete loss of activity, no proton pumping | Vigna radiata |
D279E | complete loss of activity, no proton pumping | Vigna radiata |
D283A | complete loss of activity, no proton pumping | Vigna radiata |
D283E | complete loss of activity, no proton pumping | Vigna radiata |
D287A | complete loss of activity, no proton pumping | Vigna radiata |
D287E | complete loss of activity, no proton pumping | Vigna radiata |
D723A | complete loss of activity, no proton pumping | Vigna radiata |
D723E | complete loss of activity, no proton pumping | Vigna radiata |
D727A | complete loss of activity, no proton pumping | Vigna radiata |
D727E | complete loss of activity, no proton pumping | Vigna radiata |
D731A | complete loss of activity, no proton pumping | Vigna radiata |
D731E | complete loss of activity, no proton pumping | Vigna radiata |
E263D | 50% of hydrolytic activity, no proton pumping | Vigna radiata |
E263G | complete loss of activity, no proton pumping | Vigna radiata |
K261R | 25% of hydrolytic activity, no proton pumping | Vigna radiata |
V259A | 60% of hydrolytic activity, no proton pumping | Vigna radiata |
General Stability | Organism |
---|---|
Mg2+ partially stabilizes enzyme against tryptic digestion | Vigna radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | insensitive to KF | Vigna radiata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuolar membrane | - |
Vigna radiata | 5774 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity and stability | Vigna radiata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
SDS-PAGE | Vigna radiata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | Vigna radiata | important for energy metabolism, provides energy for biosynthetic reactions, reaction coupled with proton pumping through the vacuole membrane | 2 phosphate + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O + H+[side 1] | - |
Vigna radiata | 2 phosphate + H+[side 2] | - |
? | |
diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, reaction coupled with proton pumping through the vacuole membrane | Vigna radiata | 2 phosphate + H+[side 2] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
H+-translocating inorganic pyrophosphatase | - |
Vigna radiata |