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Literature summary for 7.1.3.1 extracted from

  • McIntosh, M.T.; Vaidya, A.B.
    Vacuolar type H+ pumping pyrophosphatases of parasitic protozoa (2002), Int. J. Parasitol., 32, 1-14.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
K+ stimulates activity Trypanosoma cruzi
K+ stimulates activity Plasmodium falciparum
K+ stimulates activity Thermotoga maritima
K+ stimulates activity Toxoplasma gondii
additional information insensitive to stimulation with K+ Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
E263D 50% of hydrolytic activity, no protein translocation Vigna radiata
K261R 25% of hydrolytic activity, no protein translocation Vigna radiata
V259A 60% of hydrolytic activity, no protein translocation Vigna radiata
V262A no effect on activity Vigna radiata

General Stability

General Stability Organism
Mg2+ protects against inactivation and protease digestion Rhodospirillum rubrum

Inhibitors

Inhibitors Comment Organism Structure
bisphosphonates weak inhibition Plasmodium falciparum
bisphosphonates
-
Toxoplasma gondii
bisphosphonates
-
Trypanosoma cruzi
risedronate IC50: 0.123 mM Plasmodium falciparum
risedronate IC50: 0.002 mM Toxoplasma gondii
risedronate IC50: 0.123 mM Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
acidocalcisome
-
Trypanosoma cruzi 20022
-
membrane
-
Plasmodium falciparum 16020
-
membrane
-
Rhodospirillum rubrum 16020
-
membrane
-
Thermotoga maritima 16020
-
membrane
-
Pyrobaculum aerophilum 16020
-
plasma membrane
-
Trypanosoma cruzi 5886
-
plasma membrane
-
Toxoplasma gondii 5886
-
vacuolar membrane
-
Zea mays 5774
-
vacuolar membrane
-
Arabidopsis thaliana 5774
-
vacuolar membrane
-
Vigna radiata 5774
-
vacuolar membrane
-
Toxoplasma gondii 5774
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Zea mays
Mg2+ required Arabidopsis thaliana
Mg2+ required Trypanosoma cruzi
Mg2+ required Vigna radiata
Mg2+ required Plasmodium falciparum
Mg2+ required Rhodospirillum rubrum
Mg2+ required Thermotoga maritima
Mg2+ required Pyrobaculum aerophilum
Mg2+ required Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O + H+[side 1] Zea mays important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] Arabidopsis thaliana important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] Trypanosoma cruzi important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] Vigna radiata important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] Plasmodium falciparum important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] Rhodospirillum rubrum important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] Thermotoga maritima important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] Pyrobaculum aerophilum important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] Toxoplasma gondii important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy 2 phosphate + H+[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Plasmodium falciparum
-
hyperthermophilic bacterium
-
Pyrobaculum aerophilum
-
hyperthermophilic bacterium
-
Rhodospirillum rubrum
-
-
-
Thermotoga maritima
-
hyperthermophilic bacterium
-
Toxoplasma gondii
-
-
-
Trypanosoma cruzi
-
hyperthermophilic bacterium
-
Vigna radiata
-
mung bean
-
Zea mays
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
Plasmodium falciparum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O + H+[side 1]
-
Zea mays 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1]
-
Arabidopsis thaliana 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1]
-
Trypanosoma cruzi 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1]
-
Vigna radiata 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1]
-
Plasmodium falciparum 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1]
-
Rhodospirillum rubrum 2 phosphate + H+[side 2]
-
r
diphosphate + H2O + H+[side 1]
-
Thermotoga maritima 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1]
-
Pyrobaculum aerophilum 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1]
-
Toxoplasma gondii 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy Zea mays 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy Arabidopsis thaliana 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy Trypanosoma cruzi 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy Vigna radiata 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy Plasmodium falciparum 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy Rhodospirillum rubrum 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy Thermotoga maritima 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy Pyrobaculum aerophilum 2 phosphate + H+[side 2]
-
?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy Toxoplasma gondii 2 phosphate + H+[side 2]
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.002
-
IC50: 0.002 mM Toxoplasma gondii risedronate
0.123
-
IC50: 0.123 mM Trypanosoma cruzi risedronate
0.123
-
IC50: 0.123 mM Plasmodium falciparum risedronate