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Literature summary for 7.1.3.1 extracted from

  • Schultz, A.; Baltscheffsky, M.
    Properties of mutated Rhodospirillum rubrum H+-pyrophosphatase expressed in Escherichia coli (2003), Biochim. Biophys. Acta, 1607, 141-151.
    View publication on PubMed
Search for more literature for 7.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
D217A almost complete loss of hydrolytic and proton translocation activity Escherichia coli
D217E almost complete loss of hydrolytic and proton translocation activity Escherichia coli
D428N almost complete loss of hydrolytic and proton translocation activity Escherichia coli
E231D almost complete loss of hydrolytic and proton translocation activity Escherichia coli
E231Q almost complete loss of hydrolytic and proton translocation activity Escherichia coli
E351A 50% of hydrolytic activity, 80% of proton translocating activity Escherichia coli
E351D 30% of hydrolytic activity, 50% of proton translocating activity Escherichia coli
E351Q 55% of hydrolytic activity, 60% of proton translocating activity Escherichia coli
E384A 30% of hydrolytic activity, 55% of proton translocating activity Escherichia coli
E384D 10% of hydrolytic activity, 35% of proton translocating activity, increased tolerance to high salt concentrations Escherichia coli
K469A almost complete loss of hydrolytic and proton translocation activity Escherichia coli
K469D almost complete loss of hydrolytic and proton translocation activity Escherichia coli
K469R almost complete loss of hydrolytic and proton translocation activity Escherichia coli
R176A almost complete loss of hydrolytic and proton translocation activity Escherichia coli
R176K 50% of hydrolytic activity, 20% of proton translocating activity, increased tolerance to high salt concentrations Escherichia coli
R176K/E584D less than 10% of hydrolytic activity, no proton translocation Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 diphosphate pH 7.8, 30°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane located within or near the membrane Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 SDS-PAGE Escherichia coli
71000
-
 calculated from predicted amino acid sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O + H+[side 1] Escherichia coli important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
 isolated vesicles, pH 7.8, 30°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O + H+[side 1]
-
 Escherichia coli 2 phosphate + H+[side 2]
-
 ?
diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Escherichia coli 2 phosphate + H+[side 2]
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Escherichia coli