Crystallization (Comment) | Organism |
---|---|
purified mitochondrial membrane F1-ATPase in complex with ADP and Mg2+, 10 mg/ml protein in solution containing 200 mM Tris-DCl, pH 7.2, 400 mM NaCl, and 1 mM ADP, and prepared with D2O, mixed with precipitation solution containing 100 mM Tris-DCl, pH 7.2, 400 mM NaCl, 4 mM MgCl2, 1 mM ADP, and 14% w/v PEG 6000, followed by dialysis against 3 mL of buffer consisting of 100 mM Tris-DCl, pH 8.2, 400 mM NaCl, 4 mM MgCl2, 1 mM ADP, 5 mM phosphorous acid, and 9% w/v PEG 6000 adding PEG is 0.25% steps, room temperature, 1-4 weeks, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement and modeling | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | - |
Bos taurus | 31966 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | Bos taurus | - |
ADP + phosphate + H+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P19483 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Bos taurus | ADP + phosphate + H+/out | - |
? | |
ATP + H2O + H+/in | catalytic mechanism of F1-ATPase by structure-function relationship analysis, overview. During hydrolysis of ATP, the rotor turns counterclockwise as viewed from the membrane domain of the intact enzyme in 120 degree steps. Because the rotor is asymmetric, at any moment the three catalytic sites are at different points in the catalytic cycle. One site is devoid of nucleotide and represents a state that has released the products of ATP hydrolysis. A second site has bound the substrate, magnesium. ATP, in a precatalytic state, and in the third site, the substrate is about to undergo hydrolysis | Bos taurus | ADP + phosphate + H+/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure analysis of conformations of the betaE-, betaTP- and betaDP-subunits in ground-state structure of F1-ATPase, catalytic sites and conformational changes, overview | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
F1-ATPase | - |
Bos taurus |