Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | deletion of the extra amino acid segment of FoF1 ATPase gamma subunit results in a high ATP hydrolysis activity | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant strains with a C-terminally truncated epsilon subunit, i.e. epsilonDELTAC, or with a gamma subunit lacking the inserted sequence of residues 198-222, i.e. gammaDELTA198-222, or a double mutant of both. All mutant strains show a lower intracellular ATP level and lower cell viability under prolonged dark incubation compared with the wild-type. Thylakoid membranes from the epsilonDELTAC strain showed higher ATP hydrolysis and lower ATP synthesis activities than those of the wild-type, but no significant difference is observed in growth rate and in intracellular ATP level both under light conditions and during light-dark cycles. The maximal ATPase activity of the epsilonDELTAC mutant is 2fold or more higher than for the wild-type, both show a similar drop in activity after transfer to the dark, phenotypes, overview | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the FoF1 ATPase epsilon subunit strongly inhibits ATP hydrolysis activity | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
ATP | pH 8.0, 30°C, recombinant mutant epsilonDELTAC, ATP hydrolysis | Synechocystis sp. | |
0.14 | - |
ATP | pH 8.0, 30°C, recombinant wild-type enzyme, ATP hydrolysis | Synechocystis sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
thylakoid membrane | - |
Synechocystis sp. | 42651 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | Synechocystis sp. | - |
ADP + phosphate + H+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Synechocystis sp. | ADP + phosphate + H+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F0F1-ATPase | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
ATP hydrolysis assay at | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
ATP hydrolysis assay at | Synechocystis sp. |
General Information | Comment | Organism |
---|---|---|
malfunction | lethal phenotype of the epsilon knock-out mutant | Synechocystis sp. |
metabolism | internal inhibition of ATP hydrolysis activity of F0F1-ATP synthase is very important for cyanobacteria that are exposed to prolonged dark adaptation and, in general, for the survival of photosynthetic organisms in an ever-changing environment | Synechocystis sp. |