Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes | Bacillus sp. PS3 |
Protein Variants | Comment | Organism |
---|---|---|
alphaC193S | site-directed mutagenesis, the betaN-terminal His10-tagged mutant is constructed for bulk ATPase assays | Bacillus sp. PS3 |
gammaS107C/E165C | site-directed mutagenesis, the alphaN-terminal His6-tagged mutant is constructed for single molecule manipulation | Bacillus sp. PS3 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | inhibition mechanism, overview | Bacillus sp. PS3 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bacillus sp. PS3 | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bacillus sp. PS3 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | Bacillus sp. PS3 | - |
ADP + phosphate + H+/out | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. PS3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes | Bacillus sp. PS3 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + H2O + 4 H+[side 1] = ADP + phosphate + 4 H+[side 2] | reaction mechanism and structure-fucntion analysis, overview | Bacillus sp. PS3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Bacillus sp. PS3 | ADP + phosphate + H+/out | - |
r | |
ATP + H2O + H+/in | FoF1 is resting in the subunit epsilon-inhibited state, Fo motor must transmit to gamma subunit a torque larger than expected from thermodynamic equilibrium to initiate ATP synthesis, reaction mechanism, overview | Bacillus sp. PS3 | ADP + phosphate + H+/out | - |
r |
Synonyms | Comment | Organism |
---|---|---|
F1 | - |
Bacillus sp. PS3 |
F1-ATPase | - |
Bacillus sp. PS3 |
FoF1 ATP synthase | - |
Bacillus sp. PS3 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus sp. PS3 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | assay at | Bacillus sp. PS3 |