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Literature summary for 7.1.2.2 extracted from

  • Saita, E.; Iino, R.; Suzuki, T.; Feniouk, B.A.; Kinosita, K.; Yoshida, M.
    Activation and stiffness of the inhibited states of F1-ATPase probed by single-molecule manipulation (2010), J. Biol. Chem., 285, 11411-11417.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes Bacillus sp. PS3

Protein Variants

Protein Variants Comment Organism
alphaC193S site-directed mutagenesis, the betaN-terminal His10-tagged mutant is constructed for bulk ATPase assays Bacillus sp. PS3
gammaS107C/E165C site-directed mutagenesis, the alphaN-terminal His6-tagged mutant is constructed for single molecule manipulation Bacillus sp. PS3

Inhibitors

Inhibitors Comment Organism Structure
ADP inhibition mechanism, overview Bacillus sp. PS3

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Bacillus sp. PS3 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus sp. PS3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in Bacillus sp. PS3
-
ADP + phosphate + H+/out
-
r

Organism

Organism UniProt Comment Textmining
Bacillus sp. PS3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes Bacillus sp. PS3

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + 4 H+[side 1] = ADP + phosphate + 4 H+[side 2] reaction mechanism and structure-fucntion analysis, overview Bacillus sp. PS3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
Bacillus sp. PS3 ADP + phosphate + H+/out
-
r
ATP + H2O + H+/in FoF1 is resting in the subunit epsilon-inhibited state, Fo motor must transmit to gamma subunit a torque larger than expected from thermodynamic equilibrium to initiate ATP synthesis, reaction mechanism, overview Bacillus sp. PS3 ADP + phosphate + H+/out
-
r

Synonyms

Synonyms Comment Organism
F1
-
Bacillus sp. PS3
F1-ATPase
-
Bacillus sp. PS3
FoF1 ATP synthase
-
Bacillus sp. PS3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Bacillus sp. PS3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 assay at Bacillus sp. PS3