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Literature summary for 7.1.2.2 extracted from
- Iron transport by proteoliposomes containing mitochondrial F1Fo ATP synthase isolated from rat heart (2010), Biochimie, 92, 333-342.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP-PCP | - |
Rattus norvegicus |  |
| m-chlorophenylhydrazone | - |
Rattus norvegicus | |
| additional information | no inhibition of Fe2+ uptake and ATPase activity by ouabain at 0.1 mM, and by ATP at 1 mM | Rattus norvegicus | |
| oligomycin | a specific inhibitor of F1Fo ATP synthase, severely blocks transport of iron | Rattus norvegicus | |
| phosphate | phosphate in the medium exerted an opposite effect on iron uptake depending on the type of adenosine nucleotide, which is suppressed with ATP, but enhanced with ADP | Rattus norvegicus | |
| piceatanol | an F1 inhibitor, also inhibits Fe2+ uptake | Rattus norvegicus | |
| quercetin | an F1 inhibitor, also inhibits Fe2+ uptake | Rattus norvegicus | |
| resveratrol | an F1 inhibitor, also inhibits Fe2+ uptake | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial membrane | - |
Rattus norvegicus | 31966 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibits Fe2+ uptake by the enzyme by 25%, and the ATPase activity | Rattus norvegicus | |
| Cu2+ | inhibits Fe2+ uptake by the enzyme by 56%, and the ATPase activity | Rattus norvegicus | |
| Mg2+ | required for ATPase/ATP synthase activity, stimulates Fe2+ uptake activity | Rattus norvegicus | |
| phosphate | phosphate in the medium exerts an opposite effect on iron uptake depending on the type of adenosine nucleotide, which is suppressed with ATP, but enhanced with ADP. Mn2+ does not affect the Fe2+ uptake | Rattus norvegicus | |
| Zn2+ | inhibits Fe2+ uptake by the enzyme by 29%, and the ATPase activity slightly | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Fe2+/in | Rattus norvegicus | the enzyme transports Fe2+ and contributes to the iron uptake into rat heart. The activity of ATPase and ATP synthase may be associated with iron uptake in a different manner, probably via antiport of H+ | ADP + phosphate + Fe2+/out | - |
? | |
| ATP + H2O + H+/in | Rattus norvegicus | - |
ADP + phosphate + H+/out | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from mitochondria | Rattus norvegicus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution of the purified enzyme in proteoliposomes | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Fe2+/in | the enzyme transports Fe2+ and contributes to the iron uptake into rat heart. The activity of ATPase and ATP synthase may be associated with iron uptake in a different manner, probably via antiport of H+ | Rattus norvegicus | ADP + phosphate + Fe2+/out | - |
? | |
| ATP + H2O + H+/in | - |
Rattus norvegicus | ADP + phosphate + H+/out | - |
r | |
| additional information | the enzyme also performs slight transport of common divalent and trivalent metal ions such as Mg2+, Ca2+, Mn2+, Zn2+, Cu2+, Fe3+, and Al3+ | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| F1Fo ATP synthase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
optimum for Fe2+ uptake and ATPase activity | Rattus norvegicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
50 | activity range for Fe2+ uptake and ATPase activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | role of the F1Fo ATP synthase in iron transport, and involvement of proton-coupled transport associated with the cgamma subunit | Rattus norvegicus |
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Release 2023.1 (January 2023)
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